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- Regulatory Status
- RUO
- Other Names
- Ras-related GTP-binding protein, Ras-related protein Rab-10, GTP-binding protein RAB10, RAB-10
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- Product Citations
- publications
-
Human recombinant Phospho (Thr73) RAB10 protein (20 ng per lane) was resolved on a 12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-Rab10 Phospho (Thr73) (left panel) or purified anti-Rab10 Pan (right panel). Proteins were visualized by chemiluminescence detection using HRP Donkey anti-Rabbit (Cat. No. 406401), or HRP Goat anti-mouse IgG (Cat. No. 405306). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 560607 | 200 µg | 400€ | ||||
Member of the RAB family of small GTPases, RAB10 is a key regulator of intracellular membrane trafficking. Specifically, RAB10 plays a crucial role in the transport of proteins and recycling of endosomes to the plasma membrane. Regulation of RAB10 involves cycling between an active GTP-bound state and an inactive GDP-bound state, controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). RAB10 phosphorylation at threonine 73 is primarily mediated by Leucine-rich repeat kinase 2 (LRRK2) which plays a crucial role in regulating its interactions with various effector proteins. Dysregulation of RAB10 has been implicated in various diseases, including Charcot-Marie-Tooth disease, a hereditary neurological disorder, and certain types of cancer such as astrocytoma. Additionally, alterations in RAB10 function are associated with neurodegenerative diseases. For example, in Parkinson’s disease, mutations on LRRK2 leads to hyperphosphorylation of RAB10, disrupts normal functions and contribute to the pathophysiology of the disease.
Product DetailsProduct Details
- Source
- Synthetic Human RAB10, amino acids 66-181 phosphorylated at threonine 73
- Molecular Mass
- The 116 amino acid recombinant protein (66-181) has a predicted molecular mass of 13.3 kD. The phospho (Thr73) RAB10 [66-181] migrates at the expected molecular weight by SDS-PAGE.
- Purity
- > 95% as determined by RP-HPLC
- Formulation
- Lyophilized from a solution of protein in H2O/MeCN 0.1% TFA
- Concentration
- 200 µg per vial
- Storage & Handling
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Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted under aseptic conditions and stored at -20°C or colder. Stock solutions can be prepared in 0.22 µm filtered DI-water and acetonitrile (1:1). It is not recommended to solubilize in PBS or Tris-HCl as the protein shows poor solubility in those buffers. It is recommended to keep the material at a concentration of 1 mg/mL as dimerization becomes more pronounced at lower concentrations.
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. The product is stable up to 1 month at room temperature but leads to dimerization. Avoid repeated freeze/thaw cycles. - Application
-
WB - Verified
- Application Notes
-
A small portion of the protein in the lyophilized sample may be dimerized (< 5%). To remove dimers, we recommend adding DTT (25 µg/mL) or TCEP (29 µg/mL). DTT should not exceed 0.5 mM to avoid peptide degradation and DTT adducts. TCEP should not be exceed 0.2 mM to avoid forming adducts originating from TCEP excess.
Storing the protein at 4°C and freeze-thaw cycles increases the chance of peptide dimerization.
Antigen Details
- Structure
- RAB10 is a small GTPase protein with a molecular weight of approximately 22.5 kDa. Structurally, RAB10 contains four highly conserved GTPase motifs and a C-terminal geranylgeranylation motif, which are crucial for its membrane association.
- Distribution
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Endoplasmic Reticulum (ER), Golgi Apparatus, Late Endosomes, and Presynaptic Terminals.
- Function
- RAB10 activity is regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). GEFs, such as DENND4C and RABIF, facilitate the activation of RAB10 by promoting the exchange of GDP for GTP. On the other hand, GAPs accelerate the hydrolysis of GTP to GDP, thereby inactivating RAB10.
- Interaction
- RAB10 interacts with kinesin motors KIF13A and KIF13B, which are important for the formation of tubular endosomes.
- Biology Area
- Cancer Biomarkers, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
- Molecular Family
- GTPases, Neurotransmitter Receptor Trafficking/Endocytosis
- Antigen References
-
- Xu S, et al. 2024. Cell Biol Toxicol. 40:28.
- Erol OD, et al. 2024. Mol Cell Biochem, 479:255-281.
- Guadagno NA, et al. 2019. Cells. 8:909.
- Taylor CA, et al. 2015. PLoS Genet. 11:e1005695.
- Xu L, et al. 2021. Biomolecules. 11:1340.
- Singh V, et al. 2023. Acta Neuropathologica Communications. 11:201.
- Gene ID
- 10890 View all products for this Gene ID
- UniProt
- View information about RAB10 Phospho THR73 on UniProt.org
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