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Purified anti-human CD158b1/j (KIR2DL2/KIR2DS2) Antibody

Pricing & Availability
Clone
S22032A (See other available formats)
Regulatory Status
RUO
Other Names
CD158b1 (KIR2DL2, p58.2), CD158j (KIR2DS2, p50.2), KIR-NKAT2
Isotype
Mouse IgG2b, κ
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Product Citations
publications
S22032A_PURE_-CD158b1-j_FC_010625
Human peripheral blood lymphocytes were stained with purified anti-human CD158b1/j (KIR2DL2/KIR2DS2) (clone S22032A) (left) or purified mouse IgG2b, κ isotype control (right), followed by goat anti-mouse IgG PE. Cells were then stained with anti-human CD56 (clone QA17A16) APC.
  • S22032A_PURE_-CD158b1-j_FC_010625
    Human peripheral blood lymphocytes were stained with purified anti-human CD158b1/j (KIR2DL2/KIR2DS2) (clone S22032A) (left) or purified mouse IgG2b, κ isotype control (right), followed by goat anti-mouse IgG PE. Cells were then stained with anti-human CD56 (clone QA17A16) APC.
Cat # Size Price Save
317614 100 µg ¥63,360
Description

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. Fifteen polymorphic KIR genes have been reported in humans.

CD158b1 and CD158j, also known as KIR2DL2 and KIR2DS2, respectively, are glycoproteins with two extracellular Ig domains. KIR2DL2 displays a long cytoplasmic tail with ITIM motifs and is 38 kD. KIR2DS2, with a short cytoplasmic tail containing an ITAM motif, is 33 kD in size.  Both KIR2DL2 and KIR2DS2 are expressed primarily on a subset of NK cells and a subset of T cells. KIR2DL2 and KIR2DS2 have been reported to bind to certain HLA-C antigens on a target cell (HLA-C*01, HLA-C*03, HLA-C*07, for example).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human KIR2DL2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S22032A does not block the binding of clone DX27 on target cells and is not blocked by DX27.

Based on in-house testing on different KIR-expressing transfectants, clone S22032A does not interact with other KIR protein family members.

RRID
AB_3683335 (BioLegend Cat. No. 317614)

Antigen Details

Structure
KIR2DL2 is 38 kD and has two extracellular Ig-like domains.
KIR2DS2 is 33 kD and has two extracellular Ig-like domains.
Distribution

Subset of NK cells, subset of T cells

Function
NK cell regulation
Ligand/Receptor
Some HLA-C molecules
Cell Type
Lymphocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References
  1. Vilches C, et al. 2002. Annu Rev Immunol. 92.107361.
  2. Middleton D, et al. 2010. Immunol. 123:8.
  3. Wisniewski A, et al. 2012. Human Immu. 73.9.
  4. Lin Z, et al. 2016. Cell Reports. 17.9.
Gene ID
3803 View all products for this Gene ID 100132285 View all products for this Gene ID
UniProt
View information about CD158b1/j on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/06/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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