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Purified anti-GDF3 Antibody

Pricing & Availability
Clone
A21102G (See other available formats)
Regulatory Status
RUO
Other Names
Growth/differentiation factor 3, GDF-3
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A. A21102G_PURE_-GDF3_IHC-P_1_031325
IHC staining with Purified anti-GDF3 (clone A21102G) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-GDF3 (clone A21102G) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • A. A21102G_PURE_-GDF3_IHC-P_1_031325
    IHC staining with Purified anti-GDF3 (clone A21102G) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-GDF3 (clone A21102G) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • B. A21102G_PURE_-GDF3_IHC-P_2_031325
    IHC staining of Purified anti-GDF3 (clone A21102G) on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-GDF3 (clone A21102G) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
Cat # Size Price Save
672951 25 µg ¥37,400
672952 100 µg ¥92,400
Description

GDF3 (Growth/Differentiation Factor 3) is a member of the transforming growth factor-beta (TGF-β) superfamily, and is involved in regulating stem cell differentiation, tissue patterning, and embryonic development. GDF3 is particularly important in the development of the early embryo, influencing mesoderm and ectoderm formation, and left-right axis patterning. In stem cells GDF3 has been shown to modulate pluripotency, with its expression being critical for maintaining the undifferentiated state. GDF3 also interacts with other signaling pathways, including those of BMPs (bone morphogenetic proteins), to fine-tune cellular processes such as bone formation. In adults, GDF3 is involved in wound healing, the inflammatory response, and skeletal muscle remodeling.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human GDF3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

This product is not recommended for use in Western Blot (WB), immunocytochemistry (ICC) or intracellular flow cytometry (ICFC).

Antigen Details

Structure
GDF3 is a 364 amino acid protein with a predicted molecular weight of 41.4 kD.
Distribution

Cytoplasmic, secreted

Function
Growth factor
Ligand/Receptor
TGFBR1, TGFBR2, ALK7
Biology Area
Cell Biology, Stem Cells
Molecular Family
Growth Factors
Antigen References
  1. Levine AJ, Brivanlou AH. 2006. Cell Cycle. 5:1069-73.
  2. Levine AJ, Brivanlou AH. 2006. Development. 133:209-16. 
  3. Bisgrove BW, et al. 2017. Elife. 6:e28534.
  4. Chen C, et al. 2006. Development. 133:319-29.
Gene ID
9573 View all products for this Gene ID
UniProt
View information about GDF3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/13/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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