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Purified anti-Desmin Antibody

Pricing & Availability
Clone
W23114A (See other available formats)
Regulatory Status
RUO
Other Names
Desmin (DES)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
A. W23114A_PURE_Desmin_IHC-P_031925
IHC staining with Purified anti-Desmin (clone W23114A) on formalin-fixed paraffin-embedded human heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 10X (Cat. No. 420902). The tissue was incubated without (panel A) and with (panel B) Purified anti-Desmin (clone W23114A), followed by incubation with Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 20X objective. Scale bar: 50 µm
  • A. W23114A_PURE_Desmin_IHC-P_031925
    IHC staining with Purified anti-Desmin (clone W23114A) on formalin-fixed paraffin-embedded human heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 10X (Cat. No. 420902). The tissue was incubated without (panel A) and with (panel B) Purified anti-Desmin (clone W23114A), followed by incubation with Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 20X objective. Scale bar: 50 µm
  • B. W23114A_PURE_Desmin_ICC_031925
    Cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) and permeabilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. HeLa cells (negative control, panel A) and RD cells (positive control, panel B) were then stained with Purified anti-Desmin (clone W23114A), followed by incubation with Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 60X objective. Scale bar: 50 µm
  • C. W23114A_PURE_Desmin_WB_031925
    Whole cell extracts (15 µg total protein) from indicated cell lines and tissue were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Desmin (clone W23114A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • D. W23114A_PURE_Desmin_ICFC_031925
    RD cells (positive control, filled histogram) and HeLa cells (low expression control, open histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with Purified anti-Desmin (clone W23114A), or Purified rat IgG1, κ Isotype Control (Cat No. 400402) (open histogram, dashed line) (representative histogram for both cell lines)
Cat # Size Price Save
672851 25 µg ¥38,500
672852 100 µg ¥100,100
Description

Desmin is an intermediate filament protein found primarily in muscle cells, where it plays a critical role in maintaining the structural integrity and organization of the cell. Desmin forms a network that connects myofibrils to the sarcolemma and cytoskeleton, providing mechanical support during muscle contraction. This ensures proper alignment and function of the sarcomere, the basic contractile unit of muscle. Desmin also plays a role in muscle mitochondrial dynamics, as loss of Desmin results in defects in mitochondrial morphology, location, and function.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of the N-terminus of human Desmin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). 

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:

  • Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
  • Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
  • 100% ice-cold methanol only

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803), True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Signal is brightest using True-Nuclear™ Transcription Factor Buffer Set.

Antigen Details

Structure
Desmin is a 470 amino acid protein with a predicted molecular weight of 53.5 kD.
Distribution

Cytoskeletal

Function
Intermediate filament
Interaction
Dystrophin, alpha-actinin, plectin, titin, vimentin
Biology Area
Cardiovascular Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure
Molecular Family
Intermediate Filaments
Antigen References
  1. Hnia K, et al. 2015. Cell Tissue Res. 360:591-608.
  2. Lazarides E and Hubbard BD. 1976. Proc Natl Acad Sci U S A. 73:4344-8. 
  3. Carlsson L and Thornell LE. 2001. Acta Physiol Scand. 171:341-8. 
  4. Paulin D and Li Z. 2004. Exp Cell Res. 301:1-7.
Gene ID
1674 View all products for this Gene ID
UniProt
View information about Desmin on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All Desmin Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-Desmin W23114A IHC-P,ICC,ICFC,WB
Go To Top Version: 1    Revision Date: 03/19/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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