Loading...

Brilliant Violet 421™ Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Brilliant Violet 421™
Ave. Rating
Submit a Review
Product Citations
publications
SAV_BV421_071911
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
  • SAV_BV421_071911
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
See Brilliant Violet 421™ spectral data
Cat # Size Price Save
405226 100 µL ¥42,460
405225 100 µg ¥101,640
Description

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 421™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 421™ by 405 nm laser light induces a fluorescence maximum emission of 421 nm.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Cat. No. 405225: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Cat. No. 405226: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
Streptavidin was conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
405225 is bottled at 0.5 mg/ml.
405226 is bottled at 0.1 mg/ml.
(concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested
ICC, IHC-F, IHC-P
Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 421™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 421™ conjugation. For immunofluorescent staining, we recommend using Cat. No. 405226 at dilutions ≤ 0.015 µg in 100 µL staining volume per million cells. For applications requiring high concentration of streptavidin reagent such as tetramer labeling, we recommend using Cat. No. 405225. It is recommended that the reagent be titrated for optimal performance for each application.

* Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Streptavidin-Brilliant Violet 421™ is useful as a second step reagent for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Brilliant Violet 421™ is 340 kD and Streptavidin alone is 52 kD.

Additional Product Notes

View more applications data using this product in immunofluorescence microscopy and labeling tetramers in flow cytometry in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Kim G, et al. 2013. J. Immunol. 190:1510. PubMed.
  2. Giltiay NV, Et al. 2013. J. Immunol. 191:2155. PubMed
  3. Sugiyama D, et al. 2013. PNAS. 110:17945. PubMed
  4. Scally SW, et al. 2013. J. Exp Med. 210:2569. PubMed
  5. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  6. Higdon LE, et al. 2014. PNAS. 111:5652. PubMed
  7. Friedman RS, et al. 2014. PNAS. 111:9223. PubMed
  8. van de Weijer ML, et al. 2014. Nat Commun. 5:3832. PubMed
  9. Mise-Omata S, et al. 2014. Int Immunol. PubMed
  10. Choo JA, et al. 2014. J Virol. 88:10613. PubMed
  11. Xing YL, et al. 2014. J Neurosci. 34:14128. PubMed
  12. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  13. Poh Cm, et al. 2014. Infect Immun. 82:4854. PubMed
  14. Cabrera-Perez J, et al. 2015. J Immunol. 194:1609. PubMed
  15. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  16. Krishnaswamy JK, et al. 2015. PNAS. 112:3056. PubMed
  17. Rampuria P, et al. 2015. Int Immunol. 27:253. PubMed
Product Citations
  1. Angkasekwinai P, et al. 2017. PLoS One. 10.1371/journal.pone.0184684. PubMed
  2. van Eeden C, et al. 2018. Cell Rep. 25:3123. PubMed
  3. Combes AJ, et al. 2022. Cell. 185:184. PubMed
  4. He T, et al. 2022. Liver Int. 42:1287. PubMed
  5. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  6. Wolf EW, et al. 2022. Proc Natl Acad Sci U S A. 119:e2201794119. PubMed
  7. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  8. Lei X, et al. 2022. J Exp Med. 219: . PubMed
  9. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  10. Wang Z, et al. 2022. J Exp Med. 219: . PubMed
  11. Buckner CM, et al. 2022. Cell. 185:4333. PubMed
  12. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  13. Scharf L, et al. 2023. JCI Insight. 8: . PubMed
  14. Touizer E, et al. 2023. iScience. 26:105862. PubMed
  15. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  16. Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed
  17. Guo Q, et al. 2023. Front Public Health. 10:1067342. PubMed
  18. Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed
  19. Zhang S, et al. 2023. J Clin Med. 12: . PubMed
  20. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  21. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  22. Sun P, et al. 2022. iScience. 25:105202. PubMed
  23. Tan GMY, et al. 2022. Cancers (Basel). 14:. PubMed
  24. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
  25. Kawai H, et al. 2023. JBMR Plus. 7:e10722. PubMed
  26. Nihei Y, et al. 2023. Sci Adv. 9:eadd6734. PubMed
  27. Marcial-Juárez E, et al. 2023. iScience. 26:106310. PubMed
  28. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  29. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  30. Lu T, et al. 2023. J Med Virol. 95:e28730. PubMed
  31. Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed
  32. Sigmund EC, et al. 2023. PLoS One. 18:e0285597. PubMed
  33. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  34. Reyes RA, et al. 2021. PLoS One. 16:e0261656. PubMed
  35. Steines L, et al. 2021. Front Immunol. 12:657894. PubMed
  36. García-Alegria E, et al. 2021. STAR Protoc. 2:100367. PubMed
  37. Takizawa F, et al. 2016. J Immunol. 196: 4522 - 4535. PubMed
  38. Rad S M AH, et al. 2020. PLoS One. 15:e0232915. PubMed
  39. Miura M, et al. 2019. Wellcome Open Res. 3:105. PubMed
  40. Li H, et al. 2022. Front Immunol. 13:988004. PubMed
  41. Chaugule S, et al. 2021. Int J Mol Sci. 22:. PubMed
  42. Hayashi M, et al. 2017. EBioMedicine. 15:127-136. PubMed
  43. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  44. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  45. Parra–Sánchez H, et al. 2019. Viruses. 1.153472222. PubMed
  46. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  47. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  48. Xiong W, et al. 2021. Int J Mol Sci. 22:. PubMed
  49. Xing Y, et al. 2014. J Neurosci. 34:14128. PubMed
  50. Sei J, et al. 2016. PLoS One. 11: 0152192. PubMed
  51. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  52. Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed
  53. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  54. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  55. Mitra T, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.06.035. PubMed
  56. Poon MML, et al. 2021. Sci Immunol. 6:eabl9105. PubMed
  57. Trivedi S, et al. 2015. Vaccine. 33: 7315-7327. PubMed
  58. Sugiyama D, et al. 2013. Proc Natl Acad Sci U S A. 110:17945. PubMed
  59. Giltiay N, et al. 2013. J Immunol. 191:2155. PubMed
  60. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  61. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  62. Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed
  63. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  64. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  65. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  66. Gaudette BT, et al. 2021. J Clin Invest. 131:. PubMed
  67. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  68. Im S, et al. 2016. Nature. 537:417-421. PubMed
  69. Poh C, et al. 2014. Infect Immun . 82:4854. PubMed
  70. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  71. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  72. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  73. Zhang R, et al. 2021. MAbs. 13:1904546. PubMed
  74. Koay H, et al. 2016. Nat Immunol. 10.1038/ni.3565. PubMed
  75. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  76. Neumann B, et al. 2019. Cell Stem Cell. 25:473. PubMed
  77. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  78. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  79. Krishnaswamy J, et al. 2015. Proc Natl Acad Sci U S A. 112:3056. PubMed
  80. Cameron G, et al. 2015. J Immunol. 195: 4604 - 4614. PubMed
  81. Rowan A, et al. 2016. PLoS Pathog. 12:e1006030. PubMed
  82. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  83. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  84. Tarke A, et al. 2022. Cell. 185:847. PubMed
  85. Bähr A, et al. 2016. PLoS One. 11: 0155676. PubMed
  86. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  87. Liu B, et al. 2015. J Immunol. 194:3583. PubMed
  88. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  89. Cavazzoni CB, et al. 2022. Cell Rep. 38:110399. PubMed
  90. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  91. Roberts E, et al. 2016. PLoS One. 11:e0168488. PubMed
  92. Elayeb R, et al. 2016. Haematologica. 101: 209 - 218. PubMed
  93. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  94. Weijer M, et al. 2014. Nat Commun. 5:3832. PubMed
  95. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  96. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  97. Song R, et al. 2022. Front Immunol. 13:1028246. PubMed
  98. Borroto A, et al. 2022. Front Immunol. 13:799919. PubMed
  99. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  100. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  101. Sanjo H, et al. 2015. PLoS One. 10: 0128761. PubMed
  102. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
  103. Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed
  104. Salzberger W, et al. 2015. PLoS One. 10: 0145324. PubMed
  105. Tsuzuki S, et al. 2015. Int Immunol. 10.1093/intimm/dxv058. PubMed
  106. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  107. Scally S, et al. 2013. J Exp Med. 210:2569. PubMed
  108. Blomberg R, et al. 2019. Matrix Biol. 83:60. PubMed
  109. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  110. Wang Z, et al. 2022. J Exp Med. 219:. PubMed
  111. Guelen L, et al. 2022. J Immunother Cancer. 10:. PubMed
  112. Fra-Bido S, et al. 2021. STAR Protocols. 2(3):100499. PubMed
  113. Claudepierre M, et al. 2014. J Virol. 88:5242. PubMed
  114. Friedman R, et al. 2014. Proc Natl Acad Sci U S A. 111:9223. PubMed
  115. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  116. Wen J, et al. 2020. Cell Rep. 31:107566. PubMed
  117. Tulyeu J, et al. 2021. Curr Protoc. 1:e283. PubMed
  118. Lederer K, et al. 2022. Cell. . PubMed
  119. Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
  120. Dan JM, et al. 2021. Science. :371. PubMed
  121. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
  122. An X, et al. 2017. PLoS One.. 10.1371/journal.pone.0181904. PubMed
  123. Fries CN, et al. 2021. Sci Rep. 11:14494. PubMed
  124. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  125. Lang P 2015. Int Immunol. 27:253. PubMed
  126. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
  127. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  128. Zhu L, et al. 2016. J Cell Sci. 129: 4238 - 4251. PubMed
  129. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  130. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  131. Wells DK, et al. 2020. Cell. 183:818. PubMed
  132. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  133. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  134. Clement RL, et al. 2019. Nat Immunol. 20:1360. PubMed
  135. Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
  136. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  137. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  138. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  139. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  140. Choo J, et al. 2014. J Virol. 88:10613. PubMed
  141. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  142. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  143. Cho A, et al. 2022. J Exp Med. 219:. PubMed
  144. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
  145. Giri T, et al. 2022. Heliyon. 8:e09407. PubMed
  146. Higdon L, et al. 2014. Proc Natl Acad Sci U S A. 111:5652. PubMed
  147. Kim G, et al. 2013. J Immunol. 190:1510. PubMed
  148. Binnewies M, et al. 2019. Cell. 177:556. PubMed

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 8    Revision Date: 12/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account