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Purified anti-human CD158b/j (KIR2DL2/L3/S2) Antibody

Pricing & Availability
Clone
S22038E (See other available formats)
Regulatory Status
RUO
Other Names
CD158b1 (KIR2DL2, p58.2, NKAT6), CD158b2 (KIR2DL3, p58.3, NKAT2), CD158j (KIR2DS2, p50.2), KIR-NKAT2
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
S22038E_PURE_CD158bj_FC_122424
Human peripheral blood lymphocytes were stained with purified anti-human CD158b/j (KIR2DL2/L3/S2) (clone S22038E) (left) or purified rat IgG1, κ isotype control (right), followed by goat anti-rat IgG PE. Cells were then stained with anti-human CD56 (clone QA17A16) APC.
  • S22038E_PURE_CD158bj_FC_122424
    Human peripheral blood lymphocytes were stained with purified anti-human CD158b/j (KIR2DL2/L3/S2) (clone S22038E) (left) or purified rat IgG1, κ isotype control (right), followed by goat anti-rat IgG PE. Cells were then stained with anti-human CD56 (clone QA17A16) APC.
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316810 100 µg 78€
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Description

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. Fifteen polymorphic KIR genes have been reported in humans.

CD158b1, CD158b2, and CD158j are 33-38 kD two extracellular Ig domain glycoprotein that displays a long cytoplasmic tail with ITIM motifs for KIR2DL2/KIR2DL3 or a short cytoplasmic tail with an ITAM motif for KIR2DS2. It is expressed primarily on a subset of NK cells and a subset of T cells. KIR2DL2/KIR2DS2/KIR2DL3 has been reported to bind to certain HLA-C antigens on a target cell (HLA-C*01, HLA-C*03, HLA-C*07, for example).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human KIR2DS2 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S22038E is able to block the binding of clone DX27 on target cells and is partially blocked by DX27.

Based on in-house testing on different KIR-expressing transfectants, clone S22038E does not interact with other KIR protein family members.

Antigen Details

Structure
33-38 kD two Ig-like extracellular domain glycoprotein
Distribution

Subset of NK cells, subset of T cells

Function
NK cell regulation
Ligand/Receptor
Some HLA-C molecules
Cell Type
Lymphocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References
  1. Vilches C, et al. 2002. Annu Rev Immunol. 92:107361.
  2. Middleton D, et al. 2010. Immunol. 123:8.
  3. Moesta AK, et al. 2012. Frontiers Immu. 3:336.
  4. David G, et al. 2013. Journal of Immu. 191:9.
Gene ID
3803 View all products for this Gene ID 3804 View all products for this Gene ID 100132285 View all products for this Gene ID
UniProt
View information about CD158b CD158j on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/27/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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