Loading...

Purified anti-mouse CD16/32 Antibody

Pricing & Availability
Clone
93 (See other available formats)
Regulatory Status
RUO
Other Names
Fcγ R III/II, Ly-17
Isotype
Rat IgG2a, λ
Ave. Rating
Submit a Review
Product Citations
publications
93_purified_051906
C57BL/6 mouse splenocytes stained with purified 93, followed by anti-rat IgGs FITC
  • 93_purified_051906
    C57BL/6 mouse splenocytes stained with purified 93, followed by anti-rat IgGs FITC
Compare all formats
Cat # Size Price Quantity Check Availability Save
101301 50 µg 29€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
101302 500 µg 92€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Sorted pre-B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.

The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 µg per 106 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered).

Application References
  1. Personal communication (IP)
  2. Oliver AM, et al. 1999. Hybridoma 18:113. (Block)
  3. Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
  4. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (Block)
  5. Clements JL, et al. 2006. J. Immunol. 177:905.
  6. Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed
  7. Ouchi T, et al. 2011. J. Exp Med. 208:2607. PubMed
  8. Kmieciak M, et al. 2011. J. Vis. Exp. 47:2381. PubMed
  9. Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed
  10. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  11. Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed
  12. Koon HW, et al. 2013. J. Vis. Exp. 68:4208. PubMed
  13. Hegde VL, et al. 2013. J Biol Chem. 288:36810. PubMed
  14. Huang J, et al. 2013. J. Immunol Methods. 387:254. PubMed
  15. Dutow P, et al. 2014. J Infect Dis. PubMed
  16. Fan Y, et al. 2014. J Exp Med. 211:313. PubMed
  17. Huang HN, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed
  18. Takei S, et al. 2014. Vaccine. 32:3066. PubMed
  19. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  20. Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed
  21. Kimura T, et al. 2014. Int Immunol. 26:697. PubMed
  22. Everad A, et al. 2014. Nat Commun. 5:5648. PubMed
  23. Cenci E, et al. 2006. J. Leuko. Biol. 79(1):40-5. (Block)
Product Citations
  1. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  2. Chida J, et al. 2020. PLoS Pathog. 16:e1008823. PubMed
  3. Iyer S, et al. 2021. Cancer Discov. 0.725. PubMed
  4. Park E, et al. 2021. Journal of Neuroscience Research. . PubMed
  5. Gabitova-Cornell L, et al. 2020. Cancer Cell. 38(4):567-583.e11. PubMed
  6. Kosti P, et al. 2021. Cell Reports Medicine. 2(4):100227. PubMed
  7. Barkaway A, et al. 2021. Immunity. . PubMed
  8. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  9. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  10. Foster A, et al. 2021. EBioMedicine. 71:103571. PubMed
  11. Kiepura A, et al. 2021. Int J Mol Sci. 22:. PubMed
  12. Rajagopalan A, et al. 2021. Cell Rep Methods. 1:. PubMed
  13. Guo H, et al. 2020. Curr Protoc Immunol. 131:e107. PubMed
  14. Van Braeckel-Budimir N, et al. 2021. J Immunother Cancer. 9:. PubMed
  15. Wang Z, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  16. Xiang G, et al. 2022. Cell Death Dis. 13:451. PubMed
  17. Nederlof R, et al. 2022. Front Immunol. 13:908023. PubMed
  18. Cerrato G, et al. 2021. Cell Death Differ. 28:1733. PubMed
  19. Da Mesquita S, et al. 2021. Nature. 593:255. PubMed
  20. Liu C, et al. 2022. Nat Nanotechnol. 17:531. PubMed
  21. Lee J, et al. 2015. Hum Reprod. 10.1093/humrep/dev222. PubMed
  22. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  23. Filippini D, et al. 2019. Sci Rep. 9:12286. PubMed
  24. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  25. Sade-Feldman M,et al. 2017. Nat Commun.. 10.1038/s41467-017-01062-w. PubMed
  26. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  27. Gan T, et al. 2019. Front Microbiol. 9:3174. PubMed
  28. Furuhashi K, et al. 2017. Immunology. 151:227. PubMed
  29. Fomin ME, et al. 2017. Cell Med. 0.45625. PubMed
  30. Jebbawi F, et al. 2021. Parasite Immunol. 43:e12834. PubMed
  31. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  32. Herz J, et al. 2021. Neuron. 109:3609. PubMed
  33. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  34. Glynn RA, et al. 2022. J Immunol. 208:371. PubMed
  35. Kozuki S, et al. 2022. Genes Cells. 27:192. PubMed
  36. Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed
  37. Guttman O, et al. 2022. J Cell Biol. 221: . PubMed
  38. Miyao T, et al. 2022. Elife. 11: . PubMed
  39. Yin K, et al. 2022. Genes Dev. 36:533. PubMed
  40. Malhi H, et al. 2022. Cell Rep Med. 3:100658. PubMed
  41. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  42. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  43. Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed
  44. Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed
  45. Li X, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:1077. PubMed
  46. Akalu YT, et al. 2022. Elife. 11: . PubMed
  47. Zhang X, et al. 2022. EBioMedicine. 84:104275. PubMed
  48. Bottermann K, et al. 2022. Basic Res Cardiol. 117:48. PubMed
  49. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  50. McNamara NB, et al. 2023. Nature. 613:120. PubMed
  51. Miró L, et al. 2022. Cancers (Basel). 14: . PubMed
  52. Liu P, et al. 2023. Clin Immunol. 246:109212. PubMed
  53. Ullah I, et al. 2023. Cell Rep Med. 4:100893. PubMed
  54. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  55. Cousin N, et al. 2022. Cells. 12: . PubMed
  56. Peng Q, et al. 2023. EMBO Rep. 24:e56034. PubMed
  57. Tichet M, et al. 2023. Immunity. 56:162. PubMed
  58. Zhou H, et al. 2023. Front Immunol. 13:1059687. PubMed
  59. Mandour MF, et al. 2023. Front Med (Lausanne). 9:1057252. PubMed
  60. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  61. Novaj A, et al. 2023. Int J Mol Sci. 24: . PubMed
  62. Ishikawa T, et al. 2023. Bio Protoc. 13:e4588. PubMed
  63. Liu X, et al. 2022. Pain. 163:508. PubMed
  64. Zhang M, et al. 2022. Mol Imaging Biol. 24:280. PubMed
  65. Zhang W, et al. 2022. Dev Cell. 57:329. PubMed
  66. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  67. Chen K, et al. 2022. Adv Sci (Weinh). 9:e2104106. PubMed
  68. Zhang H, et al. 2022. Nat Cancer. 3:808. PubMed
  69. Fang Y, et al. 2022. Sci Immunol. 7:eabp9962. PubMed
  70. Koch MS, et al. 2022. Mol Ther Oncolytics. 26:275. PubMed
  71. Harapas CR, et al. 2022. Sci Immunol. 7:eabi4611. PubMed
  72. Ding P, et al. 2022. Elife. 11:. PubMed
  73. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  74. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
  75. Melo-Silva CR, et al. 2022. Cell Rep. 41:111676. PubMed
  76. Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed
  77. Wu Q, et al. 2023. Front Immunol. 13:1081719. PubMed
  78. Li L, et al. 2023. iScience. 26:106095. PubMed
  79. Torrente D, et al. 2023. J Neuroinflammation. 20:56. PubMed
  80. Bai X, et al. 2023. Nat Commun. 14:1247. PubMed
  81. Rocca Y, et al. 2023. Methods Mol Biol. 2618:187. PubMed
  82. Dang YZ, et al. 2023. Neoplasma. 70:123. PubMed
  83. Senatus L, et al. 2023. Commun Biol. 6:280. PubMed
  84. Kudo T, et al. 2023. iScience. 26:106342. PubMed
  85. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  86. Zhang YN, et al. 2023. Nat Commun. 14:1985. PubMed
  87. Iguchi A, et al. 2023. iScience. 26:106375. PubMed
  88. Feng Y, et al. 2023. J Transl Med. 21:290. PubMed
  89. Ji Y, et al. 2023. Nat Cell Biol. 25:726. PubMed
  90. Liu JC, et al. 2023. Int J Mol Sci. 24:. PubMed
  91. Petenkova A, et al. 2023. Nat Commun. 14:2761. PubMed
  92. Xu J, et al. 2023. Discov Oncol. 14:68. PubMed
  93. An R, et al. 2022. Cell Insight. 1:100047. PubMed
  94. Wang K, et al. 2023. Nat Commun. 14:2950. PubMed
  95. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  96. Li M, et al. 2023. BMC Cancer. 23:497. PubMed
  97. Mawambo G, et al. 2023. J Neuroinflammation. 20:145. PubMed
  98. Akasaka H, et al. 2023. JCI Insight. 8:. PubMed
  99. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  100. Correa S, et al. 2020. ACS Nano. 14:2224. PubMed
  101. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  102. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  103. Benitez AA, et al. 2020. Commun Biol. 0.371527778. PubMed
  104. Castoldi Â, et al. 2020. Nat Commun. 3.310416667. PubMed
  105. Mevel R, et al. 2020. eLife. 9:00. PubMed
  106. Golden GJ, et al. 2021. MBio. 12:e0118121. PubMed
  107. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  108. Huang Y, et al. 2021. Nat Immunol. 22:586. PubMed
  109. MacLean M, et al. 2022. Commun Biol. 5:177. PubMed
  110. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  111. Fan Y, et al. 2014. J Exp Med. 211:313. PubMed
  112. Berry CT, et al. 2020. Cell Reports. 31(2):107474.. PubMed
  113. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  114. Biljes D, et al. 2017. EXCLI J. 0.86875. PubMed
  115. Zhang R, et al. 2019. Cell Rep. 28:2647. PubMed
  116. McGill BE et al. 2018. The Journal of Neuroscience. 38(1):200-219 . PubMed
  117. Zou B, et al. 2017. PLoS Pathogens. 13(6):e1006436. PubMed
  118. Kitagawa S, et al. 2022. NPJ Vaccines. 7:115. PubMed
  119. Browning D, et al. 2017. Gene Ther. 10.1038/gt.2016.88. PubMed
  120. Morris MG, et al. 2020. Comp Med. 70:336. PubMed
  121. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  122. Martos-Rus C, et al. 2021. Sci Rep. 11:2974. PubMed
  123. Liu P, et al. 2021. Front Med (Lausanne). 8:625130. PubMed
  124. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  125. Jiang W, et al. 2021. Oncol Lett. 22:625. PubMed
  126. Khamissi FZ, et al. 2022. Sci Adv. 8:eabm5900. PubMed
  127. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  128. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  129. Feng J, et al. 2021. iScience. 24:103004. PubMed
  130. Wu L, et al. 2022. Front Immunol. 13:860761. PubMed
  131. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  132. Bhatia D, et al. 2019. JCI Insight. 4:e132826. PubMed
  133. Noh JR, et al. 2018. Sci Rep. 8:15222. PubMed
  134. Flores M, et al. 2008. FASEB J. 3.459027778. PubMed
  135. Tong J, et al. 2011. J Immunol. 186:3686. PubMed
  136. Tan H,et al. 2017. Mucosal Immunol. . 10.1038/mi.2017.89. PubMed
  137. Clarke F,et al. 2017. PLoS One. 10.1371/journal.pone.0186625. PubMed
  138. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
  139. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  140. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  141. Takeda Y, et al. 2018. Innate Immun. 24:323. PubMed
  142. Goel S, et al. 2017. Nature. 548:471. PubMed
  143. Matsui S, et al. 2018. Am J Pathol. 188:2059. PubMed
  144. Bauer KM, et al. 2022. JCI Insight. 7:. PubMed
  145. Liang F, et al. 2021. Front Immunol. 12:658715. PubMed
  146. Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
  147. Clark AL, et al. 2021. Diabetes Obesity and Metabolism. :. PubMed
  148. Stahnke S, et al. 2021. Current Biology. 31(10):2051-2064.e8. PubMed
  149. Yang SB, et al. 2021. Cancers (Basel). 13:00. PubMed
  150. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  151. Zhang Y, et al. 2022. Cancers (Basel). 14:. PubMed
  152. Mirshahi F, et al. 2022. Cell Rep. 38:110454. PubMed
  153. Sun SJ, et al. 2022. Commun Biol. 5:867. PubMed
  154. Chen X, et al. 2021. Cell Death Differ. 28:1237. PubMed
  155. Roberto Raúl SG, et al. 2022. Breast Cancer (Auckl). 16:11782234211065154. PubMed
  156. Matthias J, et al. 2020. J Clin Invest. 130:4587. PubMed
  157. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  158. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  159. Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed
  160. Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed
  161. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  162. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  163. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  164. Gawden‐Bone CM et al. 2018. Immunity. 49(3):427-437 . PubMed
  165. Tyagi AM et al. 2018. Immunity. 49(6):1116-1131 . PubMed
  166. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  167. Diao L, et al. 2022. iScience. 25:105511. PubMed
  168. Hazama D, et al. 2020. Cell Chemical Biology. 27(9):1181-1191.e7. PubMed
  169. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
  170. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  171. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  172. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
  173. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  174. Godbersen-Palmer C, et al. 2020. J Immunol. 204:2973. PubMed
  175. Yang C, et al. 2020. J Control Release. 326:324. PubMed
  176. Liu D, et al. 2021. JCI Insight. 6:. PubMed
  177. Armaka M, et al. 2022. Genome Med. 14:78. PubMed
  178. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  179. Carlson P, et al. 2019. Nat Cell Biol. 21:238. PubMed
  180. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  181. Guan H, et al. 2012. PLoS One. 7:e35650. PubMed
  182. Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
  183. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  184. Fiala GJ, et al. 2019. J Immunol. 203:569. PubMed
  185. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  186. Kitashima DY, et al. 2018. EBioMedicine. 27:293. PubMed
  187. Li Y, et al. 2018. J Immunol. 201:2910. PubMed
  188. Shen H, et al. 2022. Nat Commun. 13:6030. PubMed
  189. Ma J, et al. 2021. Aging (Albany NY). 13:7211. PubMed
  190. Yu M, et al. 2021. J Clin Invest. 131:. PubMed
  191. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  192. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  193. Tal MC, et al. 2020. MBio. 11:00. PubMed
  194. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  195. Kovacs L, et al. 2020. PLoS One. 15:e0240455. PubMed
  196. Vackova J, et al. 2020. Int J Mol Sci. 21:00. PubMed
  197. Wang Y, et al. 2022. Front Immunol. 13:767630. PubMed
  198. Terrazas LI, et al. 2005. Int J Parasit. 35:1349. PubMed
  199. Bhattacherjee A, et al. 2019. Commun Biol. 2:450. PubMed
  200. Lieschke S, et al. 2019. Cells. 0.420833333. PubMed
  201. Sereni L, et al. 2018. J Allergy Clin Immunol. 142:1272. PubMed
  202. Fransen F, et al. 2017. Front Immunol. 1.295138889. PubMed
  203. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  204. Wlodarska M et al. 2017. Cell host & microbe. 22(1):25-37 . PubMed
  205. Cheng XS, et al. 2020. J Cell Mol Med. 24:8391. PubMed
  206. Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed
  207. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  208. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  209. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  210. Sheller-Miller S, et al. 2021. Sci Adv. 7:. PubMed
  211. Mao W, et al. 2021. Int J Mol Sci. 22:. PubMed
  212. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  213. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  214. Cong M, et al. 2021. Methods Mol Biol. 2388:59. PubMed
  215. Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
  216. Weiner AI, et al. 2019. NPJ Regen Med. 4:17. PubMed
  217. Russell SL, et al. 2019. Cell Rep. 29:3564. PubMed
  218. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  219. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  220. Damle SR, et al. 2018. Allergy. 73:125. PubMed
  221. Wang L, et al. 2017. Front Immunol.. 10.3389/fimmu.2017.01754. PubMed
  222. Fiaturi N, et al. 2018. J Cell Commun Signal. 314:L127. PubMed
  223. Barajas‐Mora EM et al. 2018. Molecular cell. 73(1):48-60 . PubMed
  224. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  225. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  226. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  227. Finn J et al. 2019. Cell reports. 26(11):2942-2954 . PubMed
  228. Barger SR, et al. 2019. Nat Commun. 10:1249. PubMed
  229. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  230. Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed
  231. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  232. Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed
  233. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  234. Wong HS, et al. 2021. Cell. . PubMed
  235. Kusuyama J, et al. 2021. Cell Metabolism. 33(5):939-956.e8. PubMed
  236. Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed
  237. Ruf B, et al. 2021. Cancer Immunol Res. 9:1024. PubMed
  238. Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed
  239. Hu G, et al. 2022. Cell Death Dis. 13:640. PubMed
  240. Sun Y, et al. 2022. Nat Commun. 13:3916. PubMed
  241. Gereke M, et al. 2012. J Vis Exp. 70: 4322. PubMed
  242. MacConmara M, et al. 2011. J Leukoc Biol. 89:137. PubMed
  243. Cassidy LD, et al. 2018. Autophagy. 1.455555556. PubMed
  244. Fransen F, et al. 2017. Front Immunol. 8:154. PubMed
  245. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  246. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
  247. McLaughlin PA et al. 2019. PLoS Pathog. 15(7):e1007847 . PubMed
  248. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  249. Go MJ, et al. 2018. Mol Med Rep. 13:e0194423. PubMed
  250. McGrath-Morrow S, et al. 2017. Cytokine. 10.1016/j.cyto.2017.06.002. PubMed
  251. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  252. Hu J, et al. 2020. Theranostics. 7.154166667. PubMed
  253. Baasch S, et al. 2021. Cell. . PubMed
  254. Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed
  255. Zheng J, et al. 2020. bioRxiv. . PubMed
  256. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  257. Dang Y, et al. 2021. J Cancer. 12:7158. PubMed
  258. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  259. Pishesha N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  260. Chin AL, et al. 2021. Nat Commun. 12:5138. PubMed
  261. Hong CH, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:925. PubMed
  262. Everard A, et al. 2014. Nat Commun. 5:5648. PubMed
  263. Francis M, et al. 2017. Toxicol Sci. 155(1):182-195. PubMed
  264. Deng M, et al. 2020. Nat Commun. 11:2193. PubMed
  265. Hashimoto M, et al. 2019. FASEB J. 33:8069. PubMed
  266. Csóka B, et al. 2018. JCI Insight. 38:200. PubMed
  267. Proto JD et al. 2018. Immunity. 49(4):666-677 . PubMed
  268. Rashidi M, et al. 2019. J Immunol. 203:736. PubMed
  269. Baier FA, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:745. PubMed
  270. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  271. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  272. Hubbard JJ, et al. 2020. J Exp Med. :217. PubMed
  273. Xu S, et al. 2021. Immunity. . PubMed
  274. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  275. Dieterich LC, et al. 2020. Angiogenesis. 1.24375. PubMed
  276. De Santi C, et al. 2022. Mol Ther Nucleic Acids. 29:643. PubMed
  277. Pereira M, et al. 2022. Cell Rep. 40:111225. PubMed
  278. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  279. Chen Y, et al. 2022. Front Immunol. 13:835690. PubMed
  280. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  281. Welte T, et al. 2021. iScience. 24:103349. PubMed
  282. Duvigneau S, et al. 2016. Sci Rep. 6:37045. PubMed
  283. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  284. Moon H, et al. 2020. Nat Commun. 4.270138889. PubMed
  285. Kulkarni U, et al. 2019. Mucosal Immunol. 12:545. PubMed
  286. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  287. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  288. Roy K, et al. 2019. Immunity. 50:616. PubMed
  289. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  290. Lehmann GL, et al. 2020. J Exp Med. 217:00:00. PubMed
  291. Zhao L, et al. 2020. EBioMedicine. 60:103020. PubMed
  292. Riedelberger M, et al. 2020. Bio Protoc. e3552:10. PubMed
  293. Yan W, et al. 2021. Front Med (Lausanne). 8:752223. PubMed
  294. Sun C, et al. 2022. J Adv Res. 35:71. PubMed
  295. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  296. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  297. Sundararaj K, et al. 2021. Immunology. 162:418. PubMed
  298. Dutow P, et al. 2014. J Infect Dis. 209:1269. PubMed
  299. Huang H, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed
  300. Peng L, et al. 2019. Mol Med Rep. 19:2876. PubMed
  301. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  302. Matsumoto K, et al. 2017. PLoS One. 12(6):e0175406. PubMed
  303. Sauma D, et al. 2017. Immunol Res. 10.1007/s12026-017-8936-9. PubMed
  304. Gil-Pulido J, et al. 2017. PLoS One.. 10.1371/journal.pone.0181947. PubMed
  305. Yu L, et al. 2019. Elife. 8:e46677. PubMed
  306. Chen JC, et al. 2018. Front Immunol. 0.665277778. PubMed
  307. Jeong M, et al. 2022. JCI Insight. 7:. PubMed
  308. Hu CF, et al. 2021. Front Immunol. 12:638381. PubMed
  309. Zheng J, et al. 2020. Nature. . PubMed
  310. Unudurthi SD, et al. 2020. Life Sci. 117440:247. PubMed
  311. Yamakawa D, et al. 2021. Cell Reports. 34(10):108817. PubMed
  312. Troutman TD, et al. 2021. STAR Protocols. 2(1):100363. PubMed
  313. Li X, et al. 2020. Nat Commun. 4.877777778. PubMed
  314. Chu A, et al. 2021. J Reprod Immunol. 145:103309. PubMed
  315. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  316. Moon S, et al. 2021. J Exp Med. 218:. PubMed
  317. Siggs O, et al. 2016. Proc Natl Acad Sci U S A. 113: 3706 - 3715. PubMed
  318. Seki T, et al. 2015. J Biochem. 158: 485 - 495. PubMed
  319. Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed
  320. Hegde V, et al. 2013. J Biol Chem. 288:36810. PubMed
  321. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  322. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  323. Huang BR, et al. 2020. Int J Mol Sci. 21:00. PubMed
  324. Lindborg JA, et al. 2021. Cell Reports. 34(9):108777. PubMed
  325. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  326. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  327. Yu H, et al. 2021. Front Oncol. 11:736882. PubMed
  328. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  329. Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed
  330. Babagana M, et al. 2021. Aging (Albany NY). 13:19207. PubMed
  331. Kmieciak M, et al. 2011. J Vis Exp. 47: 2381. PubMed
  332. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  333. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  334. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
  335. Enomoto D, et al. 2017. PLoS One.. 10.1371/journal.pone.0183584. PubMed
  336. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  337. Zeng J, et al. 2018. J Immunol. 200:3407. PubMed
  338. Hsu BE et al. 2019. Cell Rep. 27(13):3902-3915 . PubMed
  339. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  340. Rebecca S Dickinson et al. 2017. Wellcome open research. 0.155555556 . PubMed
  341. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  342. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  343. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
  344. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  345. Vilcaes AA, et al. 2020. Int J Mol Sci. :21. PubMed
  346. Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed
  347. Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
  348. Lehmann ML, et al. 2020. Brain Behav Immun. 88:735. PubMed
  349. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
  350. Takahashi F, et al. 2022. iScience. 25:104278. PubMed
  351. Saveljeva S, et al. 2022. Cell Metab. 34:106. PubMed
  352. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
  353. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  354. Takei S, et al. 2014. Vaccine. 32:3066. PubMed
  355. Constantinides M, et al. 2011. J Immunol. 187:309. PubMed
  356. Lenert R 2005. J Immunol. 174:2429. PubMed
  357. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  358. Spranger S, et al. 2017. Cancer Cell. 31:711. PubMed
  359. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  360. Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed
  361. Shibamoto M, et al. 2019. Int Heart J. 60:944. PubMed
  362. Matsuda T, et al. 2022. iScience. 25:105324. PubMed
  363. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  364. Liu P, et al. 2020. Mol Cell. 748:77. PubMed
  365. Zhang Y, et al. 2020. Cancer Res. 80:3345. PubMed
  366. Bi J, et al. 2022. Front Microbiol. 13:927122. PubMed
  367. Zhang MH, et al. 2020. Front Immunol. 11:560. PubMed
  368. Lyle C, et al. 2019. Sci Rep. 9:20257. PubMed
  369. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  370. Mandour MF, et al. 2020. Infect Agent Cancer. 15:30. PubMed
  371. Kolter J, et al. 2020. Immunity. 50(6):1482-1497. PubMed
  372. Nakav S, et al. 2009. Nephrol Dial Transplant. 25:931. PubMed
  373. Miranda-Hernandez S, et al. 2011. J Immunol. 187:791. PubMed
  374. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
  375. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  376. Tellechea M, et al. 2018. J Immunol. 200:305. PubMed
  377. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  378. Tomimatsu M, et al. 2022. Sci Rep. 12:16656. PubMed
  379. López-Sanz C, et al. 2022. STAR Protoc. 3:101755. PubMed
  380. Chida K, et al. 2021. Biol Pharm Bull. 44:1551. PubMed
  381. Saxena M, et al. 2020. Cancer Res. 80:3631. PubMed
  382. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  383. Alshetaiwi H, et al. 2020. Methods Mol Biol. 2236:177. PubMed
  384. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  385. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  386. Lee SA, et al. 2021. Cells. 10:. PubMed
  387. Ouchi T, et al. 2011. J Exp Med. 208:2607. PubMed
  388. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  389. Forster R, et al. 2019. FASEB J. 33:11210. PubMed
  390. Heinen A, et al. 2019. Mol Ther. 27:46:00. PubMed
  391. Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed
  392. William M, et al. 2018. J Immunol. 200:4102. PubMed
  393. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  394. Miura Y et al. 2018. eLife. 7 pii: e36572. PubMed
  395. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  396. Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
  397. Senatus L, et al. 2020. JCI Insight. 5:00. PubMed
  398. Matsumoto M, et al. 2021. Cell Host Microbe. 29(6):930-940.e4. PubMed
  399. Jin HS, et al. 2020. Cancer Immunol Res. 8:912. PubMed
  400. Sakurai Y, et al. 2021. Pharmaceutics. 13:. PubMed
  401. Banskota S, et al. 2021. Inflamm Bowel Dis. 27:914. PubMed
  402. Magaletta ME, et al. 2022. Nat Commun. 13:457. PubMed
  403. Tzeng TT, et al. 2022. NPJ Vaccines. 7:60. PubMed
  404. Kimura T, et al. 2014. Int Immunol. 26:697. PubMed
  405. Howe C, et al. 2016. Sci Rep. 6:28699. PubMed
  406. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  407. Deng M, et al. 2019. J Exp Med. 216:2838. PubMed
  408. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  409. Clements J, et al. 2006. J Immunol. 177:905. PubMed
  410. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  411. Freeman SA et al. 2018. Cell. 172(1-2):305-317 . PubMed
  412. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
  413. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  414. Rojo R, et al. 2019. Nat Commun. 10:3215. PubMed
  415. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  416. Qiu Z, et al. 2020. Cancer Genet. 245:6. PubMed
  417. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  418. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  419. Luo X, et al. 2021. Neuron. 109:2691. PubMed
  420. McDonald MM, et al. 2021. Cell. 184(5):1330-1347.e13. PubMed
  421. Alam MM, et al. 2021. eneuro. 8(1):. PubMed
  422. Sitapara RA, et al. 2020. Mol Med. 26:63. PubMed
  423. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  424. Dong H, et al. 2022. Nat Metab. 4:90. PubMed
  425. Kim K, et al. 2015. Infect Immun . 83:1150. PubMed
  426. Penzo M, et al. 2010. J Immunol. 184:4497. PubMed
  427. Mehrpouya-Bahrami P, et al. 2019. J Biol Chem. 294:7669. PubMed
  428. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  429. Blaszczak AM, et al. 2019. J Immunol. 202:2451. PubMed
  430. Sivaram N, et al. 2019. J Clin Invest. 130. PubMed
  431. Litwinoff EMS, et al. 2017. Obes Res Clin Pract. 12:174. PubMed
  432. Burl RB et al. 2018. Cell metabolism. 28(2):300-309 . PubMed
  433. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  434. Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed
  435. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  436. Phadke MS, et al. 2021. Cancer Immunol Res. 9:554. PubMed
  437. Sibler E, et al. 2021. Cells. 10:. PubMed
  438. Qin J, et al. 2020. Cancer Immunol Res. 8:1426. PubMed
  439. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  440. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  441. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  442. Sibler E, et al. 2022. Cancers (Basel). 14:. PubMed
  443. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  444. Melero I, et al. 2015. Cancer Res. 75:497. PubMed
  445. Hegde V, et al. 2015. J Immunol. 194:5211. PubMed
  446. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  447. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  448. Lyons DO, et al. 2018. PLoS One. 13:e0207704. PubMed
  449. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
  450. Effern M, et al. 2020. Immunity. 53(3):564-580.e9. PubMed
  451. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  452. Richardson M, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  453. Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed
  454. Malinczak CA, et al. 2019. J Immunol. 10.4049/jimmunol.1900741. PubMed
  455. Wang X, et al. 2018. Front Immunol. 9:2615. PubMed
  456. Kabir AU, et al. 2018. JCI Insight. 3. PubMed
  457. Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed
RRID
AB_312800 (BioLegend Cat. No. 101301)
AB_312801 (BioLegend Cat. No. 101302)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Function
Low affinity receptors for IgG
Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Unkeless JC. 1989. J. Clin. Invest. 83:355.
3. Qiu WQ, et al. 1990. Science 248:732.

Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD16/32
Specificity Alt (DOES NOT SHOW ON TDS):
CD16/32
App Abbreviation (DOES NOT SHOW ON TDS):
FC,IP,Block
Hidden Names (DOES NOT SHOW ON TDS):
Ly17, Ly 17, Fcgamma, Fcg R, FcgR, CD32/16, 2.4G2, CD16, CD32, Macrophage, Neutrophil, NK Cell
UniProt
View information about CD16/32 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/23/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account