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- Clone
- W18203A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PPP2CA, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha (PP2AA), Replication protein C (RP-C)
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
IHC staining with Purified anti-PP2A alpha (clone W18203A) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated without (panel A) and with (panel B) Purified anti-PP2A alpha (clone W18203A), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm -
IHC staining with Purified anti-PP2A alpha (clone W18203A) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No.420902), the tissue was incubated without (panel A) and with (panel B) Purified anti-PP2A alpha (clone W18203A), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 μm
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 634451 | 25 µg | 124€ | ||||
| 634452 | 100 µg | 304€ | ||||
PP2A alpha (also known as Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform or PPP2CA) is the catalytic subunit of protein phosphatase 2A (PP2A), which is involved in a wide range of cellular processes through its ability to dephosphorylate serine and threonine residues on target proteins. PPP2CA modulates the activity of proteins involved in cell cycle progression, such as cyclin-dependent kinases (CDKs), and is essential for the proper transition between different phases of the cell cycle, particularly G1/S and G2/M checkpoints. It also plays a critical role in DNA damage response, ensuring repair mechanisms are activated. Beyond cell cycle regulation, PP2A alpha influences various signaling cascades, including the MAPK, AKT, and WNT pathways, impacting cell proliferation, survival, and differentiation. Furthermore, PP2A alpha’s function in dephosphorylating tau proteins is significant in the context of neurodegenerative diseases, such as Alzheimer's disease, where abnormal phosphorylation of tau is a hallmark feature.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant full-length human PP2A alpha
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). In human kidney tissue, this antibody is recommended for use at 10 µg/mL, as non-specific staining in tubules may be observed at lower concentrations.
This product is not recommended for use in immunocytochemistry (ICC), intracellular flow cytometry (ICFC), or Western blot (WB). - RRID
-
AB_3683480 (BioLegend Cat. No. 634451)
AB_3683480 (BioLegend Cat. No. 634452)
Antigen Details
- Structure
- PP2A alpha is a 309 amino acid protein with a predicted molecular weight of 35.6 kD. Catalytic subunit of protein phosphatase 2A (PP2A).
- Distribution
-
Cytoplasmic and nuclear
- Function
- Serine/threonine phosphatase which regulates enzymes and signal transduction pathways.
- Interaction
- PP2A alpha interacts with a wide variety of proteins including AKT, c-Myc, BCL2, cyclin-dependent kinases, MAPK, CD56 and Tau
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Cell Structure, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
- Ronk H, et al. 2022. Cancer Biol Med. 19:1428-39.
- Reynhout S and Janssens V. 2019. Biochim Biophys Acta Mol Cell Res. 1866:31-50.
- Lei WL, et al. 2021. Front Cell Dev Biol. 9:638559.
- Wlodarchak N and Xing Y. 2016. Crit Rev Biochem Mol Biol. 52:162-84.
- Gene ID
- 5515 View all products for this Gene ID
- UniProt
- View information about PPSA alpha on UniProt.org
Related FAQs
Other Formats
View All PPSA alpha Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-PP2A alpha | W18203A | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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