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Purified anti-human CD20 Antibody

Pricing & Availability
Clone
W23056F (See other available formats)
Regulatory Status
RUO
Other Names
Cluster of differentiation 20 (CD20), membrane-spanning 4-domains subfamily A member 1 (MS4A1), B1, Bp35
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W23056F_PURE_CD20_IHC-P_041425
IHC staining with Purified anti-human CD20 (clone W23056F) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-human CD20 (clone W23056F) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. The tissue was also co-stained with Purified anti-human/mouse CD3ε (activation epitope) (clone APA1/1) followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature (panel C). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged (panel D). Scale bar: 50 µm
  • A. W23056F_PURE_CD20_IHC-P_041425
    IHC staining with Purified anti-human CD20 (clone W23056F) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-human CD20 (clone W23056F) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. The tissue was also co-stained with Purified anti-human/mouse CD3ε (activation epitope) (clone APA1/1) followed by incubation with Alexa Fluor® 594 Goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature (panel C). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged (panel D). Scale bar: 50 µm
  • B. W23056F_PURE_CD20_ICC_041425
    Daudi cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained without (panel A) or with Purified anti-human CD20 (clone W23056F) (panel B), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • C. B. W23056F_PURE_CD20_ICFC_041425
    Human peripheral blood lymphocytes were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with PE anti-human CD19 (clone HIB19) (Cat. No. 302208) and Purified anti-human CD20 (clone W23056F) (left), orPurified anti-rat IgG2a, κ Isotype Control (right) (Cat. No. 400501) followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
Cat # Size Price Quantity Check Availability Save
650151 25 µg 145 CHF
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650152 100 µg 390 CHF
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Description

CD20 is a cell surface protein primarily expressed on B lymphocytes which plays a key role in the regulation of B cell activation, proliferation, and differentiation. Encoded by the gene MS4A1, homo-oligomeric complexes of CD20 form calcium conductive ion channels that modulate signaling pathways necessary for B cell survival and immune responses. CD20 plays a role in B cell activation of downstream Src family kinases such as Lyn, Lck, and Fyn. Because of its selective expression on B cells CD20 has become a target for therapeutic monoclonal antibodies to treat conditions like B cell lymphomas and autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of the N terminus of human CD20 (cytosolic)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2˚C and 8˚C.
Application

IHC-P - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).

This product is not recommended for use in Western Blot.

Antigen Details

Structure
CD20 is a 297 amino acid protein with a predicted molecular weight of 33.1 kD.
Distribution

Cell membrane, B cells

Function
Calcium channel
Interaction
MHC class I and II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Morsy DE, et al. 2013. J Immunol. 191:3112.
  2. Lund FE and Randall TD. 2010. Nat Rev Immunol. 10:236.
  3. Beers SA, et al. 2010. Blood. 115:5191.
  4. Kuijpers TW, et al. 2010. J Clin Invest. 120:214.
Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.14.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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