- Clone
- 1-21 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T Cell Leukemia/Lymphoma 1
- Isotype
- Mouse IgG2b, κ
- Barcode Sequence
- ATTAACTCACCCACG
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 330511 | 10 µg | $369.00 | |||
TCL1, also known as T-cell leukemia/lymphoma protein 1A, protein p14 TCL1 and oncogene TCL-1, is a 14kD beta-barrel protein that was originally identified on the basis of its association with T-cell prolymphocytic leukemia (T-PLL). Activated by the T-cell receptor enhancer through inv(14)(q11;q32.1) or t(14;14)(q11;q32.1), TCL1 is normally expressed in immature cortical thymocytes, activated peripheral T-cells, pro-B cells, and naive mantle zone B cells of peripheral lymphoid tissues, but not in post-germinal center (GC) memory B cells and plasma cells. Akt and TCL1 interact via Akt’s pleckstrin homology domain to form hetero-oligomers at the leaflet of the inner plasma membrane stimulating the protein kinase C-mitogen activated protein kinase-extracellular signal-related kinase (PKC-MAPK-ERK) signal transduction pathway. TCL1 also inhibits activation-induced cell death in T-cells by blocking PKCθ. In addition to T-PLL dysregulation is implicated in such B-cell neoplasias as lymphoblastic lymphoma, chronic lymphocytic leukemia (CLL) and Burkitt lymphoma.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Hamster
- Immunogen
- outer α-loop region of TCL1 peptide
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ICPG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 0.5 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
Additional reported applications include: immunoprecipitation, western blot, immunohistochemical staining of frozen or formalin-fixed, paraffin embedded tissue sections.
- Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. -
Application References
(PubMed link indicates BioLegend citation) -
- Herling M, et al. 2008. Blood 111:328
- Herling M, et al. 2007. Am. J. Surg. Pathol. 31:1123
Antigen Details
- Structure
- Beta barrel protein, 14kD
- Distribution
-
Cytoplasm
- Function
- Enhances Akt mediated cell survival, stabilizes mitochondrial membrane potential
- Interaction
- Akt1, Akt2, Akt3, caco2
- Biology Area
- Cell Biology, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Narducci MG et al. 2000. Cancer Research 60:2095.
2. Teitell MA et al. 2005 Nat. Rev. Cancer 5:640
3. Laine J et al.2000. Molecular Cell 6:395
4. Virgilio L et al. 1994. Proc. Natl. Acad. Sci. 91:12530
5. Masayuk N et al. 2007 FASEB J. 21:2273
6. Despouy G et al. 2007 Blood 110:4406
7. Rhine S et al. 2006 Blood 108:1991 - Gene ID
- NA
- UniProt
- View information about TCL1 on UniProt.org
Other Formats
View All TCL1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Alexa Fluor® 647 anti-human TCL1 | 1-21 | ICFC |
| PE anti-human TCL1 | 1-21 | ICFC |
| TotalSeq™-C1486 anti-human TCL1 | 1-21 | ICPG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Alexa Fluor® 647 anti-human TCL1
Human peripheral blood lymphocytes stained with anti-CD19 (H... -
PE anti-human TCL1
Human peripheral blood lymphocytes stained with anti-CD19 (H... -
TotalSeq™-C1486 anti-human TCL1