APC anti-mouse Ig light chain λ Antibody

Pricing & Availability
Clone
RML-42 (See other available formats)
Regulatory Status
RUO
Other Names
Imunoglobulin light chain lambda
Isotype
Rat IgG2a, κ
RML-42_APC_111407
C57BL/6 mouse splenocytes stained with RML-42 APC
  • RML-42_APC_111407
    C57BL/6 mouse splenocytes stained with RML-42 APC
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Cat # Size Price Quantity Check Availability
407306 100 µg $314.00
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Description

The RML-42 monoclonal antibody reacts with immunoglobulin light chain lambda in all tested mouse haplotype (Igh-a and b). It does not react with kappa chain. The RML-42 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Ota M, et al. 2010. J. Immunol. 185:4128. PubMed
  2. Xu Z, et al. 2012. J. Immunol. 189:4470. PubMed
Product Citations
  1. Grenov A, et al. 2022. Cell Rep. 39:110778. PubMed
  2. Xu Z, et al. 2012. J Immunol. 189:4470. PubMed
  3. Peter AS, et al. 2021. Eur J Immunol. . PubMed
  4. Webster P, et al. 2018. Nat Commun. 9:2649. PubMed
  5. Blanc P, et al. 2016. Nat Commun. 7:13600. PubMed
  6. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  7. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  8. Li X, et al. 2018. Immunity. 48:530. PubMed
  9. Ota M, et al. 2010. J Immunol. 185:4128. PubMed
  10. Finkin S et al. 2019. Immunity. 51(2):324-336 . PubMed
RRID
AB_961363 (BioLegend Cat. No. 407306)
Go To Top Version: 4    Revision Date: 06/11/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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