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Ultra-LEAF™ Purified anti-human IFN-γ Recombinant Antibody

Pricing & Availability
Clone
QA21A01 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. QA21A01_ULEAF_IFN-G_ICFC_051925
Human peripheral blood cells were stimulated with Cell Activation Cocktail (with Brefeldin A) (Cat. No. 423303) for 6 hours. Cells were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set (Cat No. 426803) and intracellularly stained with Ultra-LEAF™ Purified anti-human IFN-γ recombinant (clone QA21A01) (left) or Ultra-LEAF™ Purified mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE. Cells were then stained with anti-human CD3 (clone UCHT1) APC. Data shown is gated on the lymphocyte population
  • A. QA21A01_ULEAF_IFN-G_ICFC_051925
    Human peripheral blood cells were stimulated with Cell Activation Cocktail (with Brefeldin A) (Cat. No. 423303) for 6 hours. Cells were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set (Cat No. 426803) and intracellularly stained with Ultra-LEAF™ Purified anti-human IFN-γ recombinant (clone QA21A01) (left) or Ultra-LEAF™ Purified mouse IgG1, κ isotype control (right) followed by anti-mouse IgG PE. Cells were then stained with anti-human CD3 (clone UCHT1) APC. Data shown is gated on the lymphocyte population
  • B. QA21A01_ULEAF_IFN-G_Direct-ELISA_031725
    Representative sandwich immunoassay standard curve generated Ultra-LEAF™ Purified anti-human IFN-γ recombinant (clone QA21A01) as the capture antibody, biotinylated anti-human IFN-γ (clone 4S.B3) (Cat. No. 502504) as the detection antibody and recombinant human IFN-γ (Cat. No. 570209) as the standard. TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ICFC - Quality tested
ELISA Capture, ELISPOT Capture - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is  ≤ 0.25 µg per million cells in 100 µL volume. For ELISA Capture applications, a concentration range of 0.25 - 4.0 μg/mL is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 1000 to 8 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Flow Cytometry: Clone QA21A01 does not block the staining of clones 4S.B3, B27, or W19227A, but partially blocks the staining of clone W19227C.

ELISA and ELISPOT Capture: The QA21A01 recombinant antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated 4S.B3 antibody (Cat. No. 502504) as the detecting antibody and recombinant human IFN-γ (Cat. No. 570209) as the standard. For ELISPOT capture applications, a concentration range of 0.5 – 2.0 μg/mL is recommended.

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Distribution

CD8+ and CD4+ T cells, NK cells

Function
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC. Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2.
Interaction
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press San Diego.
  2. De Maeyer E, et al. 1992. Curr Opin Immunol. 4:321-6.
  3. Farrar M, et al. 1993. Annu Rev Immunol. 11:11:571-611.
  4. Gray P, et al. 1987. Lymphokines. 13:151.
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03.17.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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