Login / Register 
United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Regulatory Status
- RUO
- Other Names
- GLcAT-P, GLcUATP, LEU7, NK-1, EC 2.4.1.135, Leu-7, HNK1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Recombinant human B3GAT1 inhibits the U-118 MG proliferation in a dose-dependent manner. ED50 is between 0.8 – 4.8 µg/mL. -
Stability Testing for Recombinant Human B3GAT1. Recombinant human hB3GAT1 was aliquoted in 20mM Tris, 150mM NaCl, pH 7.0 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to inhibit the proliferation of U-118 MG human glioblastoma cells. The ED50 for this effect is 0.8 – 4.8 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 550114 | 25 µg | 292€ | ||||
| 550116 | 100 µg | 512€ | ||||
The B3GAT1 gene encodes the glucuronosyltransferase P (GlcAT-P) enzyme, a member of the glucuronosyltransferase family, which plays a role in glycosaminoglycan (GAG) biosynthesis. This enzyme facilitates the formation of the GlcAβ1,3-Gal linkage on glycans. Substitution of Phe245 in GlcAT-P with alanine reduces the enzyme's binding affinity to its ligand. B3GAT1 is also involved in the production of the cell surface carbohydrate epitope HNK-1/CD57. The HNK-1 epitope is found as a residue on several glycoproteins and glycolipids. HNK-1-positive memory T cells exhibit impaired proliferative capacity, suggesting that this epitope may serve as a marker of immune cell senescence. The HNK-1 epitope is also detected in various cell adhesion molecules (e.g., NCAM, L1) and extracellular proteins (e.g., tenascin-R, phosphacan). B3GAT1-induced HNK-1/CD57 expression acts as a biomarker for neurological conditions such as Alzheimer's disease and psychosis. Additionally, elevated levels of B3GAT1 have been observed in human brain tissue. When combined with existing markers, HNK-1 provides a more robust diagnostic tool for neuroendocrine neoplasms (NEN). In an astrocytic tumor cell model, overexpression of B3GAT1 leads to HNK-1-positive cells. These cells exhibit ERK 1/2 inactivation, resulting in reduced proliferation in glioma cell models and smaller tumor growth in animal models compared to control groups.
Product DetailsProduct Details
- Source
- Human B3GAT1, amino acid (His25-Ile334) (Accession # Q9P2W7.2), with a N-terminal 6x His, was expressed in CHO cells.
- Molecular Mass
- The 316 amino acid recombinant protein has a predicted molecular mass of approximately 36.26 kD. The DTT-reduced and non-reduced proteins migrate at approximately 35 -62 kD and 80 – 115 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is His.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in 20mM Tris, 150 mM NaCl, pH 7.0
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Human B3GAT1 inhibits the proliferation of U-118 MG human glioblastoma cells in a dose-dependent manner. The ED50 for this effect is 0.8 - 4.8 µg/mL.
- Application
-
Biossay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Dimer
- Distribution
-
Glial cells, endothelial cells, epthelial cells and immune cells including T, B and NK cells
- Function
- B3GAT1 regulates the synthesis of glycoproteins and glycolipids that are involved in cell adhesion, ECM interaction, and signal transduction.
- Bioactivity
- Measured by its ability to inhibit the proliferation of U-118 MG human glioblastoma cells
- Cell Type
- Endothelial cells, Neurons, NK cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Death, Neurodegeneration, Neuroinflammation, Neuroscience
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Bai X, et al.1999. J Biol Chem. 274:13017-24.
- Fondeur-Gelinotte M, et al. 2007. Glycobiology. 17:857-67.
- Oka S, et al. 1992. J Biol Chem. 267:22711-4.
- Jason M Brenchley JM, et al. 2003. Blood. 101:2711-20.
- García-Ayllón MS, et al. 2017. Mol Neurobiol. 54:188-199.
- Jeffries AR, et al. 2003. Mol Psychiatry. 8:654-63.
- Fagerberg Linn, et al. 2014. Mol Cell Proteomics. 13:397-406.
- Caroli-Bottino Adriana, et al. 2020. Indian J Pathol Microbiol. 63:38-43.
- Gene ID
- 27087 View all products for this Gene ID
- UniProt
- View information about B3GAT1 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
-
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
-
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
-
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
-
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
-
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
-
Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
Follow Us