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Purified anti-mouse CCL17 (TARC) Antibody

Pricing & Availability
Clone
A20096A (See other available formats)
Regulatory Status
RUO
Other Names
CC chemokine TARC, C-C motif chemokine 17, CCL17, chemokine (C-C motif) ligand 17, small inducible cytokine subfamily A (Cys-Cys), member 17, Small-inducible cytokine A17, T cell-directed CC chemokine, TARC, Thymus and activation-regulated chemokine
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A20096A_Pure_CCL17_Antibody_1_103024-2
GM-CSF generated bone marrow derived dendritic cells (BMDCs) were treated with 1X Monensin (Cat. No. 420701) for 16-24 hours. Cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. 426803) and then intracellularly stained with purified anti-mouse CCL17 (TARC) (clone A20096A) (left) or purified rat IgG2b, κ isotype control (right) followed by anti-rat IgG PE. Cells were then stained with anti-mouse/human CD11b (clone M1/70) APC.
  • A20096A_Pure_CCL17_Antibody_1_103024-2
    GM-CSF generated bone marrow derived dendritic cells (BMDCs) were treated with 1X Monensin (Cat. No. 420701) for 16-24 hours. Cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. 426803) and then intracellularly stained with purified anti-mouse CCL17 (TARC) (clone A20096A) (left) or purified rat IgG2b, κ isotype control (right) followed by anti-rat IgG PE. Cells were then stained with anti-mouse/human CD11b (clone M1/70) APC.
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167202 100 µg 260€
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Description

CC chemokine ligand 17 (CCL17), also known as Thymus and Activation-Regulated Chemokine (TARC), was initially identified as a T cell chemokine expressed constitutively in the thymus and by PBMCs upon activation.  CCL17 has since been found to be secreted by a variety of cell types and elevated in multiple inflammatory and autoimmune conditions. CCL17-mediated recruitment of Th2 and CLA+ CD4+ T cells plays a key role in allergic diseases such as atopic dermatitis, allergic asthma, allergic rhinitis, and allergic contact dermatitis. In addition, CCL17 is significantly elevated in cases of idiopathic pulmonary fibrosis. CCL17+ DCs accumulate in atherosclerotic lesions where they are thought to limit Treg expansion. DC-derived CCL17 along with CCL22 is also implicated in the recruitment of Tregs to inflammatory sites in chronic hepatitis.  CCL17 is upregulated by TNFα, IL-4, and IFNγ in bronchial epithelial cells, TGFβ1, TNFα, and IFNγ in a human keratinocyte cell line, and histamine and prostaglandin E2P in immature DCs.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Recombinant CCL17 (aa24-93)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC – Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Chemokine
Distribution

Upon activation, CCL17 is expressed by PBMCs, monocytes, dendritic cells, bronchial epithelial cells, smooth muscle cells, keratinocytes, and fibroblasts.

Function
CCL17 chemoattracts T cells, Th2 cells, and CLA+ CD4+ T cells. It plays a major role in allergic diseases. CCL17 can be upregulated by IL-4 in T cells and by a combination of cytokines in different cells.
Interaction
T cells, Th2 cells, CLA+ CD4 T cells, T (reg) cells, NK cells, NKT cells, and macrophages
Ligand/Receptor
CCR4 (CD194)
Cell Type
Dendritic cells, Epithelial cells, Fibroblasts, Monocytes
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Imai T, et al. 1997. J. Biol. Chem. 272:15036.
  2. McIlroy A, et al. 2006. Immunology 117:507.
  3. Yogo Y, et al. 2009. Respir. Res. 10:80.
  4. Stutte S, et al. 2010. P. Natl. Acad. Sci USA 107:8736.
  5. Oo YH, et al. 2010. J. Immunol. 184:2886.
  6. Weber C, et al. 2011. J. Clin. Invest. 121:2898
Gene ID
20295 View all products for this Gene ID
UniProt
View information about CCL17 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10.30.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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