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Purified anti-Aurora A Antibody

Pricing & Availability
Clone
W23093D (See other available formats)
Regulatory Status
RUO
Other Names
Aurora Kinase A, Aurora 2, AURKA, AIK, AIRK1, ARK1, AURA, Aurora/IPL1-related kinase 1, Serine/Threonine-Protein Kinase Aurora-A
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W23093D_PURE_Aurora-A_WB_030425
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were were resolved by 4-12% Bis-Tris gel electrophoresis. HeLa cells were treated with and without Nocodazole (200 ng/mL) overnight which arrests cell cycle at G2/M phase and induces Aurora A expression. The gel was then transferred to a PVDF membrane, and probed with Purified anti-Aurora A (clone W23093D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W23093D_PURE_Aurora-A_WB_030425
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were were resolved by 4-12% Bis-Tris gel electrophoresis. HeLa cells were treated with and without Nocodazole (200 ng/mL) overnight which arrests cell cycle at G2/M phase and induces Aurora A expression. The gel was then transferred to a PVDF membrane, and probed with Purified anti-Aurora A (clone W23093D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. W23093D_PURE_Aurora-A_ICC_1_030425
    HeLa cells treated with 200 ng/mL Nocodazole overnight (panel A) or without (panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-Aurora A (clone W23093D), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 25 µm
  • C. W23093D_PURE_Aurora-A_ICC_2_030425
    Hep G2 cells were fixed and permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-Aurora A (clone W23093D), followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature (panel A). Cells were also stained with Alexa Fluor® 488 anti-Tubulin-α (Cat. No. 627906) (green, panel B) and nuclei were counterstained with DAPI (Cat. No. 422801) (blue, panel B). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 25 µm
  • d. W23093D_PURE_Aurora-A_ICFC_030425
    HeLa cells treated with Nocodazole (200 ng/mL for 24 hrs) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), then intracellularly stained with DAPI and Purified anti-Aurora A (clone W23093D) or Purified rat IgG2a, κ Isotype Control (Cat. No. 400502) followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
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687451 25 µg 136€
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687452 100 µg 344€
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Description

Aurora A (AURKA) is a serine/threonine kinase and a crucial regulator of mitosis. It is a member of the Aurora kinase family, which plays key roles in cell cycle regulation, spindle formation, and chromosome segregation. AURKA is highly conserved among eukaryotic organisms and is essential for maintaining genomic stability. Aurora A is predominantly active during the G2/M phase of the cell cycle which promotes the recruitment of pericentriolar material (PCM) to centrosomes, facilitating their maturation. Aurora A phosphorylates and activates several substrates involved in mitotic entry, such as PLK1 (Polo-like kinase 1) and CDC25B which enable the activation of cyclin-dependent kinases, driving the cell into mitosis. Aurora A stabilizes spindle microtubules by interacting with proteins such as TPX2 (Targeting Protein for Xklp2) and ensures proper bipolar spindle formation facilitating a prerequisite for accurate chromosome alignment and segregation. Aberrant expression or dysregulation of AURKA is implicated in oncogenesis. Overexpression is frequently observed in various cancers, including breast, ovarian, and colorectal cancers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human Aurora A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Fixation/permeabilization with True-Phos™ Perm Buffer (Cat. No. 425401) or Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) is not recommended.

Antigen Details

Structure
Aurora A is a 403 amino acid length protein with a predicted molecular weight of 45.8 kD and contains a Protein kinase domain.
Distribution

Localizes in the cytoplasm or in the centrosome of mitotic cells

Function
Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression and associates with the centrosome and the spindle microtubules during mitosis
Interaction
Forms complex with NEDD9 and CTTN. Interacts with CPEB1, PPP2CA.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Ubiquitin/Protein Degradation
Molecular Family
Organelle Markers, Protein Kinases/Phosphatase
Antigen References
  1. Nikonova AS, et al. 2013. Cell Mol. Life Sci.70:661-87.
  2. Mosquera JM, et al. 2013. Neoplasia. 15:1-10.
  3. Zheng F, et al. 2016. Nat Commun.7:10180.
  4. Du R, et al. 2021. Mol Cancer. 20:15.
Gene ID
6790 View all products for this Gene ID
UniProt
View information about Aurora A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.04.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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