Biotinylated Recombinant Human Nectin-1-Fc Chimera (carrier-free)

Product Details

Source

Biotinylated recombinant human Nectin-1, amino acid (Gln31-Gly346) (Accession: #Q15223.3), with a linker, C-terminal human IgG1 (Pro100-Lys300) and an -Avi tag was site-specifically biotinylated by enzyme BirA and was expressed in 293E cells.

Molecular Mass

The 568 amino acid recombinant protein has a predicted molecular mass of approximately 63.75 kD. The DTT-reduced and non-reduced proteins migrate at approximately 70 -115 kD and 140 – 240 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Gln.

Purity

> 95%, as determined by Coomassie-stained SDS-PAGE.

Formulation

0.22 µm filtered protein solution is in PBS, pH 7.2

Endotoxin Level

Less than 0.1 EU per µg of cytokine as determined by the LAL method

Concentration

25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity

When recombinant human Nectin-3 (Cat. No. 566604) is immobilized at 1 µg/mL, biotinylated recombinant human Nectin-1 binds in a dose-dependent manner with ED₅₀ range of 3.6 – 36 ng/mL. HRP-Avidin (Cat. No. 405103) was used to detect the binding.

Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure

Dimer

Distribution

Myelomonocytic cells, megakaryocytes, CD34-positive stem cells, endothelial cells, epithelial cells, and neuronal cells

Function

Adhesion, receptor for Herpes simplex Virus 1 (HSV-1) and HSV-2

Interaction

Endothelial cells, epithelial cells, hematopoietic stem and progenitors, megakaryocytes

Ligand/Receptor

Nectin-1, -2, -3, and -4, CD155, HSV-1 and HSV-2

Bioactivity

The bioactivity was measured by immobilized recombinant human Nectin-3 binding biotinylated recombinant human Nectin-1 in a dose-dependent manner using the functional ELISA.

Cell Type

Endothelial cells, Epithelial cells, Megakaryocytes, Neutrophils

Biology Area

Immuno-Oncology, Immunology, Innate Immunity

Molecular Family

Adhesion Molecules, Innate Immune Signaling

Antigen References

1. Takai Y, et al. 2003. Cancer Sci. 94:655-67
2. Takahashi K, et al. 1999. J Cell Biol. 145:8127.
3. Togashi H, et al. 2006. J Cell Biol. 174:141-51.
4. Tampakis A, et al. 2019. Oncology. 96:318-325.
5. Takai Y, et al. 2008. Nat Rev Mol Cell Biol. 9:603-15. 
6. Suzuki K, et al. 2000. Nat Genet. 25:427-30.

Gene ID

5818 View all products for this Gene ID

UniProt

View information about Nectin-1 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Certificate of Analysis