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APC/Cyanine7 anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-F11_APCCyanine7_CD45_Antibody_013019_updated.png
C57BL/6 splenocytes were stained with CD45 (clone 30-F11) APC/Cyanine7 (filled histogram) or Rat IgG2b, κ APC/Cyanine7 isotype control (open histogram).
  • 30-F11_APCCyanine7_CD45_Antibody_013019_updated.png
    C57BL/6 splenocytes were stained with CD45 (clone 30-F11) APC/Cyanine7 (filled histogram) or Rat IgG2b, κ APC/Cyanine7 isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
103115 25 µg 79€
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103116 100 µg 157€
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  2. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  3. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  4. Contijoch EJ et al. 2019. eLife. 8 pii: e40553. PubMed
  5. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  6. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  7. Ren Y, et al. 2021. Am J Transplant. 21:552. PubMed
  8. Hilfenhaus G, et al. 2021. Cancer Res. 81:619. PubMed
  9. Bruno MEC, et al. 2022. J Cell Physiol. 237:706. PubMed
  10. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  11. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  12. He D, et al. 2022. Immunity. 55:159. PubMed
  13. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  14. Goncharov NV, et al. 2022. J Evol Biochem Physiol. 58:230. PubMed
  15. Li DD, et al. 2022. J Cell Mol Med. 26:2438. PubMed
  16. Denorme F, et al. 2022. J Clin Invest. 132: . PubMed
  17. Tang Q, et al. 2022. Mol Ther. 30:2709. PubMed
  18. Chae CS, et al. 2022. Cancer Discov. 12:1904. PubMed
  19. Miyao T, et al. 2022. Elife. 11: . PubMed
  20. Serra M, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:609. PubMed
  21. Poh AR, et al. 2022. Sci Adv. 8:eabl7882. PubMed
  22. Campisi L, et al. 2022. Nature. 606:945. PubMed
  23. Kenigsbuch M, et al. 2022. Nat Neurosci. 25:876. PubMed
  24. Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed
  25. Li F, et al. 2022. Gut Microbes. 14:2096989. PubMed
  26. Rodrigues PB, et al. 2022. Cells. 11: . PubMed
  27. Zhu J, et al. 2022. Research (Wash D C). 2022:9816272. PubMed
  28. Poh AR, et al. 2022. Cell Rep. 41:111479. PubMed
  29. Zhu X, et al. 2023. Adv Mater. 35:e2207198. PubMed
  30. Li S, et al. 2022. Nat Commun. 13:7371. PubMed
  31. Morrow RJ, et al. 2022. Cells. 11: . PubMed
  32. Liu P, et al. 2023. Clin Immunol. 246:109212. PubMed
  33. Alula KM, et al. 2023. Sci Rep. 13:18. PubMed
  34. Peng Q, et al. 2023. EMBO Rep. 24:e56034. PubMed
  35. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  36. Zhou J, et al. 2023. Nutrients. 15: . PubMed
  37. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  38. Cao Y, et al. 2023. J Inflamm Res. 16:343. PubMed
  39. Tsioti I, et al. 2023. J Neuroinflammation. 20:25. PubMed
  40. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  41. Chun J, et al. 2023. Cancers (Basel). 15: . PubMed
  42. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
  43. Ishikawa T, et al. 2023. Bio Protoc. 13:e4588. PubMed
  44. Ryan CB, et al. 2022. Neurobiol Dis. 163:105608. PubMed
  45. Marsh SE, et al. 2022. Nat Neurosci. 25:306. PubMed
  46. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  47. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  48. Korsunsky I, et al. 2022. Med. 3:481. PubMed
  49. Cheng C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:261. PubMed
  50. Pattwell SS, et al. 2022. Sci Adv. 8:eabo6789. PubMed
  51. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  52. Ho HM, et al. 2022. Pharmaceutics. 14:. PubMed
  53. Cai Z, et al. 2023. Adv Sci (Weinh). 10:e2207155. PubMed
  54. Banerjee K, et al. 2023. Eur J Immunol. 53:e2250161. PubMed
  55. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  56. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  57. Liu N, et al. 2023. Cell Death Dis. 14:170. PubMed
  58. Lu C, et al. 2023. Antiviral Res. 212:105556. PubMed
  59. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  60. Saha S, et al. 2023. Sci Rep. 13:4609. PubMed
  61. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed
  62. Freitag PC, et al. 2023. Mol Ther Methods Clin Dev. 29:120. PubMed
  63. Thai LM, et al. 2023. iScience. 26:106477. PubMed
  64. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  65. Sasaki L, et al. 2022. Nat Aging. 2:105. PubMed
  66. Haensel D, et al. 2023. Nat Commun. 14:2685. PubMed
  67. Lee K, et al. 2023. JCI Insight. 8:. PubMed
  68. Wang C, et al. 2023. Int J Oral Sci. 15:19. PubMed
  69. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  70. Scieszka DP, et al. 2023. Respir Res. 24:138. PubMed
  71. Yang QC, et al. 2023. iScience. 26:106916. PubMed
  72. Ye S, et al. 2023. Front Oncol. 13:1202750. PubMed
  73. Stefkovich M, et al. 2021. Mol Metab. 54:101357. PubMed
  74. Wang Q, et al. 2020. Radiat Environ Biophys. 59:89. PubMed
  75. Kim MY, et al. 2021. JCI Insight. 6:. PubMed
  76. De Vries LCS, et al. 2021. J Crohns Colitis. 15:617. PubMed
  77. Bernasconi R, et al. 2021. EMBO Mol Med. :e14392. PubMed
  78. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  79. Da Mesquita S, et al. 2021. Nature. 593:255. PubMed
  80. Furrer R, et al. 2021. Sci Adv. 7:eabi4852. PubMed
  81. Ren D, et al. 2022. STAR Protoc. 3:101217. PubMed
  82. Chang YS, et al. 2020. Int J Mol Sci. 21:00. PubMed
  83. Naito H, et al. 2020. Nature Protocols. 15(3):1066-1081. PubMed
  84. Xing J, et al. 2021. Cell Reports. 35(12):109205. PubMed
  85. Heindl S, et al. 2021. J Exp Med. 218:. PubMed
  86. Page N, et al. 2018. Immunity. 48:937. PubMed
  87. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  88. Ajina R, et al. 2019. Oncoimmunology. 8:e1577127. PubMed
  89. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  90. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  91. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  92. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  93. Wu SY, et al. 2022. Mol Cancer. 21:84. PubMed
  94. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  95. Jiang Q, et al. 2022. Theranostics. 12:59. PubMed
  96. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  97. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  98. Urban SK, et al. 2020. Liver Int. 40:3103. PubMed
  99. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  100. Rosenkranz SC, et al. 2021. eLife. 10:00. PubMed
  101. Kokona D, et al. 2016. Exp Eye Res. S0014-4835(16)30499-7. PubMed
  102. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  103. Pluchino N, et al. 2020. J Cell Mol Med. 24:2464. PubMed
  104. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  105. Ebneter A, et al. 2017. Transl Vis Sci Technol. 10.1167/tvst.6.2.10. PubMed
  106. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  107. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  108. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  109. Zhu S, et al. 2022. Front Pharmacol. 13:996635. PubMed
  110. Palacios LM, et al. 2022. Biology (Basel). 11:. PubMed
  111. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  112. Luo R, et al. 2021. Theranostics. 11:117. PubMed
  113. Adrover JM, et al. 2022. JCI Insight. 7:. PubMed
  114. Steubing RD, et al. 2022. Brain Behav Immun Health. 24:100493. PubMed
  115. Lu H, et al. 2021. Autophagy. 17:2511. PubMed
  116. Kawashima N, et al. 2022. Nat Commun. 13:1624. PubMed
  117. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  118. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  119. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  120. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed
  121. Baram T, et al. 2021. Cells. 10:. PubMed
  122. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  123. Radovanovic I, et al. 2014. J Immunol. 193:1290. PubMed
  124. Evans H, et al. 2015. J Immunol. 195: 2973 - 2984. PubMed
  125. Cardona S, et al. 2015. ASN Neuro. 7: 1759091415608204. PubMed
  126. Han Y, et al. 2020. Nat Commun. 11:1776. PubMed
  127. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  128. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  129. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  130. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  131. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  132. Zhou X, et al. 2022. Front Pharmacol. 13:887263. PubMed
  133. Biswas S, et al. 2021. Nature. 591:464. PubMed
  134. Forte E, et al. 2020. J Cell Mol Med. . PubMed
  135. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  136. Wiley CD, et al. 2019. JCI Insight. 4:e130056. PubMed
  137. Krasteva G, et al. 2011. Proc Natl Acad Sci U S A. 108:9478. PubMed
  138. Ganeshan K et al. 2019. Cell. 177(2):399-413 . PubMed
  139. Shi S, et al. 2020. Clin Cancer Res. 26:5990. PubMed
  140. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  141. Armaka M, et al. 2022. Genome Med. 14:78. PubMed
  142. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  143. Jung BG, et al. 2022. PLoS Pathog. 18:e1010454. PubMed
  144. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  145. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
  146. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  147. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
  148. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  149. Khandelwal P, et al. 2013. PLoS One. 8:64193. PubMed
  150. Shibata K, et al. 2014. J Immunol. 192:2210. PubMed
  151. Mantia-Smaldone G, et al. 2014. Gynecol Oncol. 133:584. PubMed
  152. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  153. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  154. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  155. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  156. Ning F, et al. 2019. J Exp Med. 216:1396. PubMed
  157. Higashi AY, et al. 2019. J Am Soc Nephrol. 30:80. PubMed
  158. Lyons J, et al. 2018. Sci Signal. 11. PubMed
  159. Evans R, et al. 2019. Cell Rep. 27:1967. PubMed
  160. Hammond TR, et al. 2019. Immunity. 50:253. PubMed
  161. Schönfeld M, et al. 2021. Alcohol Clin Exp Res. 45:1980. PubMed
  162. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  163. Bagayoko S, et al. 2021. PLoS Pathog. 17:e1009927. PubMed
  164. Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed
  165. Marangoni F, et al. 2021. Cell. . PubMed
  166. Wang LT, et al. 2021. STAR Protocols. 2(1):100337. PubMed
  167. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  168. Chew W, et al. 2016. Nat Methods. 13: 868-874. PubMed
  169. Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed
  170. Weiner AI, et al. 2019. NPJ Regen Med. 4:17. PubMed
  171. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  172. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  173. Liu H, et al. 2020. Cancer Cell. 37(3):324-339. PubMed
  174. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  175. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  176. Commerford CD et al. 2018. Cell reports. . PubMed
  177. Zeng J, et al. 2019. Cell Rep. 27:549. PubMed
  178. Yousef H, et al. 2018. Bio Protoc. 8:22. PubMed
  179. Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed
  180. Donlan AN, et al. 2020. Anaerobe. 66:102275. PubMed
  181. Uher O, et al. 2021. Cancers (Basel). 13:. PubMed
  182. Liu M, et al. 2020. Cell Rep. 32:107990. PubMed
  183. Jeljeli M, et al. 2020. Cell Rep. 33:108325. PubMed
  184. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  185. Chi Z, et al. 2020. Molecular Cell. 80(1):43-58.e7. PubMed
  186. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  187. Utz B, et al. 2020. Sci Rep. 10:18733. PubMed
  188. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  189. Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed
  190. Soldevilla MM, et al. 2019. Mol Ther. 27:1878. PubMed
  191. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  192. Li Y, et al. 2019. Sci Rep. 9:6932. PubMed
  193. Mifflin L, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  194. Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
  195. Gao H, et al. 2021. Cells. 10:. PubMed
  196. Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed
  197. Ajina R, et al. 2021. Cancer Immunol Res. 9:386. PubMed
  198. Frii J, et al. 2021. Immunity. 54(5):1002-1021.e10. PubMed
  199. Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed
  200. Zhang X, et al. 2020. Elife. 9:00. PubMed
  201. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  202. Ayaub E,et al. 2017. Sci Rep.. 10.1038/s41598-017-13511-z. PubMed
  203. Peralta Ramos JM, et al. 2017. Front Immunol. 1.490277778. PubMed
  204. Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed
  205. Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
  206. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  207. Ali M, et al. 2022. JCI Insight. 7:. PubMed
  208. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  209. Cho YK, et al. 2022. Nat Commun. 13:4084. PubMed
  210. Ghandhi SA, et al. 2022. Sci Rep. 12:14124. PubMed
  211. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  212. Fu H, et al. 2020. Front Immunol. 11:595813. PubMed
  213. Oladiran O, et al. 2021. J Neuroinflammation. 77:18. PubMed
  214. Veremeyko T, et al. 2012. J Vis Exp. 65: 4097. PubMed
  215. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  216. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  217. Haikala HM, et al. 2019. Nat Commun. 10:620. PubMed
  218. Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
  219. Neuhöfer P, et al. 2021. Nature. 597:715. PubMed
  220. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  221. Hum NR, et al. 2022. Front Oncol. 12:928474. PubMed
  222. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  223. Selvaraj UM, et al. 2021. Brain Behav Immun. 95:502. PubMed
  224. Ying T, et al. 2021. Am J Physiol Endocrinol Metab. 320:E1148. PubMed
  225. Chen Y, et al. 2022. Front Immunol. 13:835690. PubMed
  226. Momin N, et al. 2022. Nat Commun. 13:109. PubMed
  227. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  228. Pilones KA, et al. 2020. Cancer Immunol Res. 8:1054. PubMed
  229. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  230. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  231. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  232. Dieterich LC, et al. 2020. Angiogenesis. 1.24375. PubMed
  233. Martina M, et al. 2014. J Vis Exp. 87: 51192. PubMed
  234. Alikhanyan K, et al. 2020. Immun Inflamm Dis. 8:181. PubMed
  235. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  236. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  237. Yang Y, et al. 2022. Elife. 11:. PubMed
  238. Dhanwani R, et al. 2020. Sci Adv. 6:eaba3688. PubMed
  239. Tripathi H, et al. 2020. Stem Cell Rev Rep. 0.953472222. PubMed
  240. Cardenas JJ, et al. 2020. Methods Enzymol. 415:631. PubMed
  241. Abramowski P, et al. 2014. J Neuroimmunol. 274:111. PubMed
  242. Hoeksema M, et al. 2015. J Immunol. 194:3909. PubMed
  243. Haka A, et al. 2012. PLoS One. 7:e44823. PubMed
  244. Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed
  245. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  246. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  247. Wang R, et al. 2022. Front Immunol. 13:947756. PubMed
  248. Becker W, et al. 2021. J Crohns Colitis. 15:1032. PubMed
  249. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  250. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  251. Clement CC, et al. 2021. Nat Commun. 12:4447. PubMed
  252. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  253. Li H, et al. 2021. Adv Sci (Weinh). 2001596:8. PubMed
  254. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  255. Alonso-Herranz L, et al. 2020. Elife. 9:00. PubMed
  256. Gierut J, et al. 2015. Stem Cell Res. 15: 165-171. PubMed
  257. Seki T, et al. 2015. J Biochem. 158: 485 - 495. PubMed
  258. Camara A, et al. 2019. Immunity. 50:1467. PubMed
  259. Michaelis KA, et al. 2019. Brain Behav Immun. 82:338. PubMed
  260. Veremeyko T, et al. 2019. J Neurosci Res. 97:162. PubMed
  261. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  262. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  263. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  264. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
  265. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  266. Yu H, et al. 2021. Front Oncol. 11:736882. PubMed
  267. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  268. Yu W, et al. 2022. Nat Commun. 13:3544. PubMed
  269. Huetter J, et al. 2020. Cancer Immunol Res. 0.954861111. PubMed
  270. Favuzzi E, et al. 2021. Cell. 184(15):4048-4063.e32. PubMed
  271. DiToro D, et al. 2020. Immunity. 650:52. PubMed
  272. Draper D, et al. 2012. J Immunol. 188:5327. PubMed
  273. Hohsfield LA, et al. 2020. J Neuroinflammation. 0.902083333. PubMed
  274. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  275. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  276. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  277. Selvaraj UM et al. 2018. Endocrinology. 159(11):3848-3859 . PubMed
  278. Pinho–Ribeiro FA, et al. 2018. Cell. 173:1083. PubMed
  279. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  280. Hsu M, et al. 2022. Nat Immunol. 23:581. PubMed
  281. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  282. Takaku S, et al. 2022. Evid Based Complement Alternat Med. 2022:6122955. PubMed
  283. Wang X, et al. 2022. Front Immunol. 13:864984. PubMed
  284. Galani IE, et al. 2022. STAR Protoc. 3:101151. PubMed
  285. Abdelfattah N, et al. 2022. Nat Commun. 13:767. PubMed
  286. Ou YC, et al. 2020. ACS Nano. 1.035416667. PubMed
  287. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  288. Zelco A, et al. 2020. Front Cell Neurosci. 0.75625. PubMed
  289. Wang LT, et al. 2020. Cell Rep. 32:108188. PubMed
  290. Hadas R, et al. 2020. JCI Insight. 5:00. PubMed
  291. Stock M, et al. 2015. Osteoarthritis Cartilage. 23: 1221-1225. PubMed
  292. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  293. Ben-Moshe S, et al. 2019. Nat Metab. 1:899. PubMed
  294. Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
  295. Kaiser T, et al. 2019. Eneuro. 6. PubMed
  296. El Agha E, et al. 2017. Cell Stem Cell. 1.014583333. PubMed
  297. Bhatt D, et al. 2021. J Exp Med. 218:. PubMed
  298. Lu Z, et al. 2021. J Clin Invest. 131:. PubMed
  299. Luo M, et al. 2021. J Immunol. 207:1683. PubMed
  300. Da Mesquita S, et al. 2021. Science Advances. 7(21):. PubMed
  301. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  302. Yu L, et al. 2020. Nat Commun. 2.045833333. PubMed
  303. Liu X, et al. 2019. Mol Cancer Ther. 18:706. PubMed
  304. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  305. Jiang P, et al. 2018. Nat Med. 24:1550. PubMed
  306. Wang X, et al. 2018. Nat Commun. 9:551. PubMed
  307. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  308. Pedicone C, et al. 2022. iScience. 25:104170. PubMed
  309. Godwin MS, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L393. PubMed
  310. Reda M, et al. 2022. Nat Commun. 13:4261. PubMed
  311. Li Y, et al. 2022. STAR Protoc. 3:101364. PubMed
  312. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  313. Milich LM, et al. 2021. J Exp Med. 218:. PubMed
  314. Kline JM, et al. 2021. J Biol Chem. :100373. PubMed
  315. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  316. Starikova E, et al. 2021. Front Pharmacol. 12:667572. PubMed
  317. Klein S, et al. 2016. J Cell Sci. 129: 2573 - 2585. PubMed
  318. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  319. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  320. Ireland L, et al. 2020. Front Immunol. 11:297. PubMed
  321. Gago-Lopez N, et al. 2019. EMBO Mol Med. 11:e10697. PubMed
  322. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  323. Uluçkan ö, et al. 2020. Cell Reports. 29(4):844-859.e3.. PubMed
  324. Armaka M, et al. 2018. Nat Commun. 9:618. PubMed
  325. Lang JF et al. 2019. Nat Commun. 10(1):3415 . PubMed
  326. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  327. Martin A, et al. 2018. Oncotarget. 9:28364. PubMed
  328. Yan C, et al. 2022. NPJ Precis Oncol. 6:6. PubMed
  329. Phansalkar R, et al. 2021. Elife. 10:. PubMed
  330. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  331. Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
  332. Lu C, et al. 2020. Cancer Cell. 39(1):96-108.e6. PubMed
  333. Yang C, et al. 2020. Cell Host Microbe. 467:27. PubMed
  334. Morein D, et al. 2021. Cells. 10: . PubMed
  335. Yoldi G, et al. 2016. Cancer Res . 76: 5857 - 5869. PubMed
  336. Ling Y, et al. 2016. Pharmacol Res. S1043-6618(16)31329-9. PubMed
  337. Willemze RA, et al. 2019. Mol Med. 25:01:00. PubMed
  338. Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed
  339. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  340. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  341. Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
  342. Wang Y, et al. 2022. Nat Commun. 13:1454. PubMed
  343. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  344. Zhang X, et al. 2022. J Exp Clin Cancer Res. 41:246. PubMed
  345. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  346. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  347. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  348. Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
  349. Wang X, et al. 2021. Theranostics. 11:209. PubMed
  350. Liu SS, et al. 2021. Immunity. :. PubMed
  351. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  352. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  353. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  354. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  355. Seeling M, et al. 2013. Proc Natl Acad Sci U S A. 110:10729. PubMed
  356. Forte E, et al. 2020. Cell Rep. 30:3149. PubMed
  357. Wang J, et al. 2020. J Hematol Oncol. 0.610416667. PubMed
  358. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  359. Petriello MC, et al. 2018. Toxicol Sci. 162:548. PubMed
  360. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  361. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  362. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  363. Sebastian A, et al. 2021. Cells. 10:. PubMed
  364. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  365. Chen MB, et al. 2020. Cell Rep. 30:4418. PubMed
  366. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  367. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  368. Cong L, et al. 2021. Breast Cancer Res. 23:51. PubMed
  369. De Micheli AJ, et al. 2020. Am J Physiol Cell Physiol. 319:C885. PubMed
  370. Sibler E, et al. 2021. Cells. 10:. PubMed
  371. Oladiran O, et al. 2021. Front Immunol. 12:720733. PubMed
  372. Lux A, et al. 2014. Cell Rep. 10:236. PubMed
  373. Madsen P, et al. 2016. J Neurosci. 36: 5128 - 5143. PubMed
  374. Fox B, et al. 2016. PLoS One. 12: 1006189. PubMed
  375. Parker K, Horn L, Ostrand-Rosenberg S 2016. J Leukoc Biol. 100: 463 - 470.. PubMed
  376. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  377. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
  378. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  379. Swanson WB, et al. 2021. Biomaterials. 272:120769. PubMed
  380. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  381. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
  382. Zhu Q, et al. 2021. J Am Heart Assoc. 10:e023491. PubMed
  383. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  384. Parent AV, et al. 2021. Cell Reports. 36(7):109538. PubMed
  385. Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
  386. Ma Y, et al. 2021. Developmental Cell. 56(4):478-493.e11. PubMed
  387. Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed
  388. Campisi L, et al. 2016. Nat Immunol. 10.1038/ni.3512. PubMed
  389. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
RRID
AB_312980 (BioLegend Cat. No. 103115)
AB_312981 (BioLegend Cat. No. 103116)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine5 anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC,IHC-F,CyTOF®,IP,CMCD,IHC,WB
PE/Cyanine7 anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC,SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC,ICC,IHC,3D IHC,SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC,SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F,FC,3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC,SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC,CyTOF®,IP,CMCD,IHC,WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Spark Blue™ 515 anti-mouse CD45 30-F11 FC
Spark UV™ 387 anti-mouse CD45 30-F11 FC
PE/Fire™ 810 anti-mouse CD45 30-F11 FC
Spark PLUS UV395™ anti-mouse CD45 30-F11 FC
Spark Red™ 718 anti-mouse CD45 30-F11 FC
Go To Top Version: 3    Revision Date: 01.30.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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