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Alexa Fluor® 647 anti-ERG Antibody

Pricing & Availability
Clone
W19256A (See other available formats)
Regulatory Status
RUO
Other Names
ETS-Related Gene, Transcriptional Regulator ERG, ETS Transcription Factor ERG, Erythroblast Transformation-Specific Transcription Factor ERG Variant 10, p55
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W19256A_A647_ERG_ICC_031225
HeLa cells (negative control, panel A) and MOLT-4 cells (positive control, panels B and C) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801), permeabilized with ice-cold methanol, and blocked with 5% FBS. The cells were then stained with Alexa Fluor® 647 anti-ERG (clone W19256A), and nuclei were counterstained with DAPI (Cat. No. 422801). Panels A and B show representative images for the DAPI and Alexa Fluor® 647 channels merged while panel C shows only the Alexa Fluor® 647 channel. Images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • a. W19256A_A647_ERG_ICC_031225
    HeLa cells (negative control, panel A) and MOLT-4 cells (positive control, panels B and C) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801), permeabilized with ice-cold methanol, and blocked with 5% FBS. The cells were then stained with Alexa Fluor® 647 anti-ERG (clone W19256A), and nuclei were counterstained with DAPI (Cat. No. 422801). Panels A and B show representative images for the DAPI and Alexa Fluor® 647 channels merged while panel C shows only the Alexa Fluor® 647 channel. Images were captured using a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • b. W19256A_A647_ERG_ICFC_031225
    U937 cells (negative control, open histogram) and MOLT-4 cells (positive control, filled histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with Alexa Fluor® 647 anti-ERG (clone W19256A) or Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat No. 400526) (open histogram, dashed line. Representative for both cell lines).
See Alexa Fluor® 647 spectral data
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940203 25 µg 148€
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Description

ERG (ETS-related gene) is a member of the erythroblast transformation-specific (ETS) family of transcription factors. Expressed in the mesoderm and endothelium of embryos, ERG is vital to embryonic cell differentiation as well as regulation of endothelial homeostasis, a role it sustains beyond the embryonic stage. Through transcriptional regulation of VE-cadherin, ICAM-2, and Claudin-5, ERG maintains endothelial junctional integrity and stability and regulation of the endothelial cytoskeleton through its interactions with HDAC6 and RhoJ. ERG is vital to angiogenesis, bone development, platelet adhesion, urogenital tract development, vascular stability, and proper hematopoiesis regulation. It plays a large role in cellular response to vascular inflammation, endothelial cell barrier function, and inhibition of vascular inflammation. These functions are accomplished largely through ERG-mediation of the Wnt/β-catenin signaling pathway by means of transcriptional regulation of FZD4 and VE-cadherin by ERG and subsequent promotion of β-catenin stability. This in turn promotes endothelial cell proliferation. ERG fusion proteins resulting from chromosomal translocations cause ERG to behave as a proto-oncogene, contributing to prostate cancer, megakaryoblastic leukemia, and Ewing’s sarcoma.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human ERG protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

For use of this antibody in Immunocytochemistry (ICC), we recommend fixation with PFA fixation Buffer (Cat. No. 420801) followed by permeabilization with either Triton X-100 (0.5 %) or ice-cold methanol.

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or True-Phos™ Perm Buffer (Cat. No. 425401).

Antigen Details

Structure
ERG is a 449 amino acid protein with an expected molecular weight of 54 kD.
Distribution

Ubiquitously expressed/ Nucleus 

Function
Transcription factor
Cell Type
Endothelial cells
Biology Area
Cell Biology, Ubiquitin/Protein Degradation
Antigen References

1. Adamo P, et al. 2016. J. Biol. Chem. 35:4013
2. Birdsey G, et al. 2015. Dev. Cell. 32:82
3. Shah A, et al. 2016. Vascul. Pharmacol. 86:3

 

Gene ID
2078 View all products for this Gene ID
UniProt
View information about ERG on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.12.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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