Brilliant Violet 421™ anti-Human/Mouse CD266 (Fn14/TWEAKR), CD266, ITEM-4

Pricing & Availability

Clone: ITEM-4 (See other available formats)
Regulatory Status: RUO
Other Names: Fibroblast growth factor-inducible 14 kD protein (Fn14), TWEAK receptor, TNFRSF12A
Isotype: Mouse IgG2b, κ
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Product Citations: publications

ITEM-4_BV421_CD266_Antibody_1_010725 — Human Fn14 transfected cells were stained with anti-human/mouse CD266 (Fn14, TWEAK R) (clone ITEM-4) Brilliant Violet 421™ (filled histogram) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).

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314109

25 tests

155€

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314110

100 tests

326€

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Description

CD266 is a 14 kD transmembrane protein known as TWEAK receptor (TWEAK-R) or fibroblast growth factor inducible 14 (Fn14). It is member 12A of the tumor necrosis factor receptor superfamily (TNFRSF12A) expressed at low levels in a variety of tissues, such as heart, placenta, lung, muscle, and pancreas. TWEAK-R binds TWEAK (CD255) and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The engagement of Fn14 can induce cell death and may play a role in inflammation, hepatocyte growth, and liver neoplasia.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Fn14 transfected P815
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting^3,4, immunofluorescence^3,4, and immunohistochemistry of frozen sections⁵ and formalin-fixed paraffin-embedded sections³. ITEM-4 antibody has no effect on TWEAK-induced MAPK activation in RAW cells¹.
RRID: AB_3683328 (BioLegend Cat. No. 314109)
AB_3683328 (BioLegend Cat. No. 314110)

Antigen Details

Structure: Transmembrane TNF superfamily receptor, contains no death domain, 14 kD
Distribution: Widely expressed in heart, placenta, lung, muscle, kidney, pancreas, and HUVEC
Function: Binds to TWEAK (CD255), thought to play a role in cell death and inflammation
Ligand/Receptor: TWEAK (CD255); interacts with cytoplasmic proteins TRAF1, 2, and 3
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Feng SL, et al. 2000. Am. J. Path. 156:1253.
2. Meighan-Mantha RL, et al. 1999. J. Biol. Chem. 274:33166.
3. Nakayama M, et al. 2003. J. Immunol. 170:341.
4. Lynch CN, et al. 1999. J. Biol. Chem. 274:8455.
Gene ID: 27279 View all products for this Gene ID 51330 View all products for this Gene ID
UniProt: View information about CD266 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

TNF Superfamily Pathway

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD266 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human/mouse CD266 (Fn14, TWEAK R)	ITEM-4	FC,ICC,IHC-P,IHC-F,WB
Biotin anti-human/mouse CD266 (Fn14, TWEAK R)	ITEM-4	FC
PE anti-human/mouse CD266 (Fn14, TWEAK R)	ITEM-4	FC
APC anti-human/mouse CD266 (Fn14, TWEAK R)	ITEM-4	FC
Brilliant Violet 421™ anti-human/mouse CD266 (Fn14, TWEAK R)	ITEM-4	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01.07.2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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